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  4. UBE2C Recombinant Monoclonal Antibody

UBE2C Recombinant Monoclonal Antibody

说明书
  • 中文名称：
    UBE2C重组抗体
  • 货号：
    CSB-RA025440MA1HU
  • 规格：
    ￥1320
  • 图片：
    • IHC image of CSB-RA025440MA1HU diluted at 1:300 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
    • IHC image of CSB-RA025440MA1HU diluted at 1:300 and staining in paraffin-embedded human stomach tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
    • Overlay Peak curve showing Hela cells stained with CSB-RA025440MA1HU (red line) at 1:100. The cells were fixed in 4% formaldehyde (15min) and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

产品详情

  • 产品描述：
    UBE2C重组单克隆抗体（CSB-RA025440MA1HU）靶向泛素结合酶E2C（UBE2C），该蛋白作为泛素-蛋白酶体系统的核心成员，通过介导底物蛋白的泛素化修饰参与细胞周期调控及有丝分裂进程，尤其在肿瘤发生发展中显示出异常表达特征。本产品经ELISA、免疫组化（IHC）和流式细胞术（FC）等多平台严格验证，在IHC应用中可清晰定位UBE2C在细胞核及胞质中的表达模式，推荐工作浓度为1:20-1:200；流式检测中同样表现出高灵敏度和特异性，适用于细胞表面及胞内抗原的定量分析。该抗体适用于探索UBE2C在肿瘤细胞增殖、DNA损伤修复及化疗耐药性中的分子机制，为癌症生物学研究、细胞周期动态示踪以及泛素化信号通路分析提供可靠工具，尤其适用于人源组织样本或细胞系的相关实验。产品经重组表达技术制备，具备批次间一致性高、交叉反应性低等优势，可满足高通量筛选及精准检测需求，适用于基础科研领域的蛋白质功能研究与分子互作分析。
  • Uniprot No.：
    O00762
  • 基因名：
    UBE2C
  • 别名：
    Cyclin selective ubiquitin carrier protein antibody; dJ447F3.2 antibody; Mitotic specific ubiquitin conjugating enzyme antibody; UB E2C antibody; UBCH 10 antibody; UbcH10 antibody; UBE 2C antibody; Ube2c antibody; UBE2C_HUMAN antibody; Ubiquitin carrier protein C antibody; Ubiquitin carrier protein E2 C antibody; Ubiquitin carrier protein E2C antibody; Ubiquitin conjugating enzyme E2 C antibody; Ubiquitin conjugating enzyme E2C antibody; Ubiquitin protein ligase C antibody; Ubiquitin-conjugating enzyme E2 C antibody; Ubiquitin-protein ligase C antibody
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human UBE2C protein
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Mouse IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    22E1
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC, FC
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:20-1:200
    FC 1:20-1:200
  • Protocols：
    ELISA Protocol
    Immunohistochemistry (IHC) Protocol
    Flow cytometry (FC) Protocol
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

产品评价

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UBE2C antibodies

UBE2C Proteins

靶点详情

  • 功能：
    Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by initiating 'Lys-11'-linked polyubiquitin chains on APC/C substrates, leading to the degradation of APC/C substrates by the proteasome and promoting mitotic exit.
  • 基因功能参考文献：
    1. UBE2C is involved in the anti-proliferative and pro-apoptotic functions of ECRG4 in esophageal squamous cell carcinoma. PMID: 29268240
    2. loss of BRCA1 function results in an increase in UBE2C expression and chemical resistance to doxorubicin in breast cancer cells. PMID: 28881292
    3. FoxM1 promotes glioma progression by enhancing UBE2C transcription PMID: 28767320
    4. SAG/RBX2 E3 ligase complexes with UBCH10 and UBE2S ubiquitin-conjugating enzymes to ubiquitylate beta-TrCP1 via K11-linkage for degradation. PMID: 27910872
    5. UBE2C and HOXA1 RNA and protein are differentially expressed in conventional and Spitz nevi and melanoma. PMID: 28657121
    6. High UBE2C expression is associated with esophageal squamous cell carcinoma. PMID: 27588470
    7. Urinary UBE2C cell-free RNA may be a valuable diagnostic marker for bladder cancer. PMID: 27528424
    8. A role was identified for UBE2C as a marker of the androgen signaling pathway in prostate cancer. PMID: 27550197
    9. Data suggest that the activity of AURKA might be regulated by UBE2C through regulating the activity of anaphasepromoting complex. UBE2C may be a new marker in the diagnosis of gastric cancer and may be a potential therapeutic target for the treatment of gastric adenocarcinoma. PMID: 28260026
    10. High UBCH10 expression is associated with bortezomib-resistance in multiple myeloma. PMID: 28000886
    11. the expression of UbcH10 in colorectal cancer samples as compared with healthy tissue sample from the same patient according to age at surgery, was examined. PMID: 26744065
    12. UbcH10 may promote gastric cancer growth. PMID: 27349176
    13. Identification of UBE2C as a target of wild-type and GOF mutant p53 further highlights the contribution of p53 in regulation of spindle assembly checkpoint PMID: 27129209
    14. The anaphase-promoting complex/cyclosome C activity in human cells is tuned by the combinatorial use of three E2 ubiquitin-conjugating enzymes, namely UBE2C, UBE2S, and UBE2D. PMID: 26904940
    15. Study shows an overexpression of UbcH10 mRNA and protein in the vast majority of colorectal cancer (CRC) patients analyzed and indicates that UbcH10 expression regulates CRC growth. PMID: 25917796
    16. the present study showed that miR-196a promoted cell proliferation by targeting UBE2C in breast cancer. Thus, miR-196a may be a potential oncogene in breast cancer and a promising therapeutic target in breast cancer treatment. PMID: 26062455
    17. UBE2C could regulate phospho-ERK1/2 level. PMID: 25832867
    18. Data suggest that ubiquitin-conjugating enzyme UbcH10 may be a promising target for the therapy of breast cancer. PMID: 25739083
    19. Upregulation of miR-17/20a promotes gastric cancer cell growth by targeting UBE2C. PMID: 25760688
    20. UbcH10 overexpression increases carcinogenesis and blocks ALLN susceptibility in colorectal cancer. PMID: 25376843
    21. in addition to binding APC11's RING, UBCH10 is corecruited via interactions with APC2 PMID: 25825779
    22. excess E2F1 due to Rb inactivation recruits the complex of Cdc20 and the anaphase-promoting complex/cyclosome to deregulate the expression of UBCH10 PMID: 25368385
    23. UBE2C may be a marker for diagnosis of nonpalpable breast lesions but not benign or malignant tumors in mammography core biopsies. Suppression of UBE2C may be a potential therapy target in breast cancer. PMID: 24699941
    24. the UBE2C gene is capable of promoting proliferation and invasion of lung cancer cells. Therefore, UBE2C may play a pivotal role in development and invasion of lung cancer. PMID: 24815438
    25. Results suggest that UBE2C acts as an oncogene in prostate cancer progression. PMID: 24512726
    26. Data suggest that ubiquitin conjugating enzyme UBE2C may serve as a potential therapeutic target aimed at inducing radiation and chemo sensitization. PMID: 24072565
    27. Elevated levels of S100A8 and UBE2C were observed. PMID: 24114735
    28. UBE2C positivity is independently associated with shorter cancer-specific survival in bladder cancer patients who underwent radical cystectomy. PMID: 23826418
    29. Data indicate that expression-based risk indices of three genes UBE2C, TPX2, and MELK were more strongly associated with poor 5-year survival in adenocarcinoma patients. PMID: 23357462
    30. High expression of UbcH10 was associated with pancreatic ductal adenocarcinoma. PMID: 23355337
    31. the expression of cyclin D1, MCM7, TRIM29, and UBE2C was found to be significantly associated with progression to muscle-invasive bladder cancer. PMID: 23201130
    32. UbcH10 overexpression has a critical role in lung carcinogenesis, and the evaluation of UbcH10 expression levels may be a new tool for the characterisation of non small cell lung carcinoma. PMID: 23102841
    33. Data sugggest that UbcH10 may play an important role in non-small cell lung cancer (NSCLC) carcinogenesis, and silencing UbcH10 may represent a potential therapeutic strategy for NSCLC. PMID: 22760214
    34. Overexpression of UbcH10 may be a useful indicator of endometrial carcinoma. PMID: 22415060
    35. growth rate of SiHa and HeLa transfected with dominant negative-UBE2C was significantly reduced compared to vector control PMID: 22694363
    36. High UBE2C mRNA expression was found to be of adverse prognostic significance in high-risk breast cancer patients PMID: 22056852
    37. UbcH10 expression was associated with tumor invasion of the portal vein, tumor size, TNM staging, and tumor differentiation in hepatocellular carcinoma. PMID: 21354912
    38. Effect of UBE2C overexpression on the proliferation of 293T cell line PMID: 21651863
    39. Mediator complex subunit (MED)1 phosphorylation leads to UBE2C locus looping, UBE2C gene expression and cell growth in castration-resistant prostate cancer. PMID: 21556051
    40. Spindle assembly checkpoint protein Cdc20 transcriptionally activates expression of ubiquitin carrier protein UbcH10. PMID: 21454660
    41. Elevated expression of UbcH10 is associated with breast cancer. PMID: 19038004
    42. elevated levels of Ubch10 and Cdc20 degrade cyclin B in hpv-16 iinfected cells, required for exit from mitosis, permitting initiation of the next round of DNA synthesis and cell cycle progression. PMID: 20739533
    43. The unstructured N-terminal region of UbcH10 directly binds the 19 S regulatory complex of the 26 S proteasome, and it mediates the initiation of substrate translocation. PMID: 20007692
    44. The overexpression of UbcH10 gene plays a critical role in the carcinogenesis and tumor progression of colorectal cancer. PMID: 19779934
    45. show that UbcH10 overexpression leads to precocious degradation of cyclin B by the anaphase-promoting complex/cyclosome, supernumerary centrioles, lagging chromosomes, and aneuploidy. PMID: 20065091
    46. Data imply that knocking-down UbcH10 protein expression may represent a potential therapeutic option for glioma. PMID: 19657671
    47. UbcH10 is highly expressed in various human primary tumors and UbcH10 has an ability to promote cell growth and malignant transformation. PMID: 12874022
    48. UbcH10 plays an important role in tumor development and that its inhibition in combination with agonists of the TRAIL receptor may provide an enhanced therapeutic index PMID: 15208666
    49. UbcH10 overexpression is involved in thyroid cell proliferation and may represent a marker of thyroid anaplastic carcinomas PMID: 16106252
    50. results showing aberrations in levels of gene expression and locus copy number of ubiquitin-conjugating enzyme E2C gene (UBE2C) suggest that this gene may play an important role in tumor progression leading to advanced colon cancer with liver metastasis PMID: 16772118

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  • 蛋白家族：
    Ubiquitin-conjugating enzyme family
  • 数据库链接：

    HGNC: 15937

    OMIM: 605574

    KEGG: hsa:11065

    STRING: 9606.ENSP00000348838

    UniGene: Hs.93002

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