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  4. THEMIS Recombinant Monoclonal Antibody

THEMIS Recombinant Monoclonal Antibody

说明书
  • 中文名称：
    THEMIS重组抗体
  • 货号：
    CSB-RA262013A0HU
  • 规格：
    ￥1320
  • 图片：
    • IHC image of CSB-RA262013A0HU diluted at 1:50 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.30% DAB.
    • Immunofluorescence staining of HepG2 with CSB-RA262013A0HU at 1:40, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 503-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Overlay Peak curve showing Jurkat cells stained with CSB-RA262013A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

产品详情

  • 产品描述：
    CSB-RA262013A0HU THEMIS重组单克隆抗体是经多种实验验证的高特异性科研工具，适用于ELISA、免疫组化（IHC）、免疫荧光（IF）及流式细胞术（FC）检测，推荐工作浓度为1:50-1:200。该抗体针对THEMIS蛋白设计，该靶标作为T细胞受体信号通路的关键调控因子，在胸腺细胞发育、T细胞活化及免疫耐受建立中发挥重要作用。经严格验证，本产品在石蜡包埋组织切片中能清晰显示细胞质定位信号，在免疫荧光实验中呈现稳定的亚细胞染色特异性，流式检测可有效识别细胞表面抗原表达，所有实验均表现出优异的批间一致性和低背景干扰。适用于研究T细胞分化机制、免疫调控网络及自身免疫性疾病模型构建，可配合多种样本类型（如冰冻/石蜡组织、原代细胞或培养细胞系）进行定量或定性分析，为免疫学基础研究及肿瘤微环境相关课题提供可靠检测方案。
  • Uniprot No.：
    Q8N1K5
  • 基因名：
    THEMIS
  • 别名：
    Protein THEMIS (Thymocyte-expressed molecule involved in selection), THEMIS, C6orf190 C6orf207
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from Human THEMIS
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    7G7
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC, IF, FC
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:50-1:200
    IF 1:50-1:200
    FC 1:50-1:200
  • Protocols：
    ELISA Protocol
    Immunohistochemistry (IHC) Protocol
    Immunofluorescence (IF) Protocol
    Flow cytometry (FC) Protocol
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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THEMIS antibodies

THEMIS Proteins

靶点详情

  • 功能：
    Plays a central role in late thymocyte development by controlling both positive and negative T-cell selection. Required to sustain and/or integrate signals required for proper lineage commitment and maturation of T-cells. Regulates T-cell development through T-cell antigen receptor (TCR) signaling and in particular through the regulation of calcium influx and phosphorylation of Erk.
  • 基因功能参考文献：
    1. we have demonstrated mutually exclusive use of exon 1 and 2 amongst 16 novel THEMIS isoforms. We also show inverse correlation between THEMIS expression in human CD4+ T-cells and dosage of the multiple sclerosis risk allele at rs13204742, driven by reduced expression of exon 1- containing isoforms PMID: 27438997
    2. we found that THEMIS directly regulated the catalytic activity of the tyrosine phosphatase SHP-1. PMID: 28250424
    3. the THEMIS:SHP complex dampens early TCR signalling by a previously unknown molecular mechanism that favours T-cell survival. PMID: 25535246
    4. THEMIS showed higher mRNA expression in active celiac disease compared with treated patients and controls. PMID: 23820479
    5. THEMIS binding to GRB2 was required for thymocyte development. PMID: 23460737
    6. Themis1 exhibits the same potential to restore T cell development and T cell receptor-mediated signaling in Themis1-deficient mice. PMID: 22732588
    7. SPOP mutations may define a new molecular subtype of prostate cancer. PMID: 22610119
    8. specific role for THEMIS in early TCR signalosome function PMID: 21189249
    9. Observational study of gene-disease association. (HuGE Navigator) PMID: 19913121
    10. Observational study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator) PMID: 20628086
    11. Observational study of gene-disease association and gene-environment interaction. (HuGE Navigator) PMID: 20095854
    12. Functional characterization of the homologous mouse gene. PMID: 19597498
    13. Functional analysis of the mouse gene by knockout, and comparison of the mouse and human proteins. PMID: 19597499

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  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    Themis family
  • 数据库链接：

    HGNC: 21569

    OMIM: 613607

    KEGG: hsa:387357

    UniGene: Hs.661756

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