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  4. SLC2A2 Recombinant Monoclonal Antibody

SLC2A2 Recombinant Monoclonal Antibody

说明书
  • 中文名称：
    SLC2A2重组抗体
  • 货号：
    CSB-RA438622A0HU
  • 规格：
    ￥1320
  • 图片：
    • IHC image of CSB-RA438622A0HU diluted at 1:50 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.20% DAB.
    • Immunofluorescence staining of HepG2 with CSB-RA438622A0HU at 1:25, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 495-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Overlay Peak curve showing HepG2 cells surface stained with CSB-RA438622A0HU (red line) at 1:50. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

产品详情

  • 产品描述：
    CSB-RA438622A0HU SLC2A2重组单克隆抗体是针对葡萄糖转运蛋白GLUT2（由SLC2A2基因编码）研发的高特异性抗体，适用于多种蛋白检测平台。该抗体经ELISA、免疫组化（IHC）、免疫荧光（IF）和流式细胞术（FC）严格验证，推荐工作浓度为IHC 1:50-1:200、IF 1:50-1:200、FC 1:50-1:200，在肝、胰腺及肠组织样本中可清晰显示GLUT2的膜定位特征。作为介导细胞内外葡萄糖转运的关键蛋白，GLUT2在糖代谢调控、胰岛素分泌及能量稳态中发挥重要作用，本产品可广泛应用于糖尿病研究、代谢性疾病模型构建、胰岛β细胞功能分析及肠道营养吸收机制探索等科研领域。采用重组单克隆技术生产，具有高批次间一致性和低交叉反应性，支持细胞、组织切片等多种样本类型检测，为体外研究GLUT2蛋白表达调控及其在生理病理过程中的功能提供可靠工具。
  • Uniprot No.：
    P11168
  • 基因名：
    SLC2A2
  • 别名：
    Solute carrier family 2, facilitated glucose transporter member 2 (Glucose transporter type 2, liver) (GLUT-2), SLC2A2, GLUT2
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from Human SLC2A2
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    37E1
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC, IF, FC
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:50-1:200
    IF 1:50-1:200
    FC 1:50-1:200
  • Protocols：
    ELISA Protocol
    Immunohistochemistry (IHC) Protocol
    Immunofluorescence (IF) Protocol
    Flow cytometry (FC) Protocol
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

产品评价

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SLC2A2 antibodies

SLC2A2 Proteins

靶点详情

  • 功能：
    Facilitative hexose transporter that mediates the transport of glucose and fructose. Likely mediates the bidirectional transfer of glucose across the plasma membrane of hepatocytes and is responsible for uptake of glucose by the beta cells; may comprise part of the glucose-sensing mechanism of the beta cell. May also participate with the Na(+)/glucose cotransporter in the transcellular transport of glucose in the small intestine and kidney. Also able to mediate the transport of dehydroascorbate.
  • 基因功能参考文献：
    1. Data suggest that the following genetic modifications are involved in neonatal diabetes mellitus patients in Oman: (1) mutation in KCNJ11 (potassium voltage-gated channel subfamily J member 11; one patient); (2) mutation in GCK (glucokinase); (3) mutation in SLC2A2 (glucose transporter type 2); (4) chromosome 6q24 methylation abnormalities. PMID: 29329106
    2. Results showed the glucose transporter GLUT2 was highly expressed in the lumen of sweat glands from atopic dermatitis (AD) patients. AD patients with chronic inflammation had significantly increased GLUT2 mRNA expression and near normal sweat glucose levels. PMID: 29677207
    3. Data suggest expression of SGLT1 is markedly increased in kidney of patients with type 2 diabetes as compared to control subjects; SGLT1 mRNA is highly and significantly correlated with fasting and postprandial plasma glucose and HbA1c. In contrast, data suggest SGLT2 and GLUT2 mRNA in kidney are down-regulated in type 2 diabetes, but not to statistically significant level. (SGLT = sodium-glucose co-transporter) PMID: 28477418
    4. The mutant tumors exhibited impaired proliferation, anoikis resistance, and migratory capability and had reduced adenylate energy charge. Further investigations also revealed that cANGPTL4 regulated the expression of Glut2 PMID: 28641978
    5. Single nucleotide polymorphism in SLC2A2 gene is associated with glycemic response to metformin in Type 2 diabetes. PMID: 27500523
    6. no significant associations between GLUT2 and/or TAS1R2 polymorphisms and fillings were found, but allele frequencies of the TAS1R2 variant were marginally significantly different between children with DMFT = 0 and DMFT >/=1. no significant interaction between both genes and risk of dental caries was found. GLUT2 and TASR1 polymorphisms may influence the risk of caries in the Czech population PMID: 26112465
    7. Three novel variants and seven single-nucleotide polymorphisms associated with the myelomeningocele phenotype. PMID: 25776730
    8. Homozygous splice-site mutation IVS8+5G>C (c.1068+5 G>C) of SLC2A2 was found in patient A and homozygous nonsense mutation c.1194T>A (p.Tyr398X) in patient B. Patient C harboured a missense mutation c.380C>A (p.Ala127Asp) PMID: 25919556
    9. Data identified the last enzyme of the de novo purine synthesis pathway 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase (ATIC)and the putative tyrosine phosphatase PTPLAD1 as new regulators of Glut2(SLC2A2)translocation in HEK293 cells. SiRNA-mediated knockdown of ATIC delayed insulin response of Glut2 translocation while depletion of PTPLAD1(HACD3} strongly enhanced it in HEK293 cells. PMID: 25687571
    10. A novel 6 nucleotide deletion in GLUT2 gene, a member of the facilitative glucose transporter family, is shown to be segregated with Fanconi-Bickel syndrome in an Iranian family. PMID: 25523092
    11. SGLT1 or GLUT2 interact with the cytoskeleton in the intestinal epithelium during hexose absorption. PMID: 25711084
    12. Mutations in the GLUT2 gene is associated with ccute metabolic acidosis in Fanconi-Bickel syndrome. PMID: 25165176
    13. GLUT-2 expression may be associated with cholangiocarcinogenesis of large bile duct and is a helpful marker for detecting high-grade biliary intraepithelial neoplasia lesions in atypical bile ducts. PMID: 24824030
    14. SGLT1 mRNA and GLUT2 mRNA expression are reduced significantly in CACo-2 cells exposed to berry extracts. PMID: 24236070
    15. the first gain of function mutations for hGLUT2, revealing the importance of its receptor versus transporter function in pancreatic beta cell development and insulin secretion. PMID: 23986439
    16. associated with caries risk PMID: 23257979
    17. This study determined if single nucleotide polymorphisms in genes involved in fructose transport,SLC2A2 and SLC2A5 and metabolism, etohexokinase affect inter-individual variability in metabolic phenotypes. PMID: 23341889
    18. Intestinal dehydroascorbic acid (DHA) transport is mediated by the facilitative sugar transporters, GLUT2 and GLUT8 PMID: 23396969
    19. Genetic variant SLC2A2 is marginally associated with risk of cardiovascular disease in type 2 diabetes mellitus patients. PMID: 23185617
    20. Mutation analysis of the GLUT2 gene in three unrelated Egyptian families with Fanconi-Bickel syndrome detected three different mutations. PMID: 22350464
    21. Case-control analyses revealed a unique association between the G allele of rs9875793 and bipolar disorder patients with 'negative mood delusions' compared with controls. PMID: 23010768
    22. GLUT2 gene expression is suppressed in Hepatitis C virus infection via downregulation of HNF-1alpha expression at transcriptional and posttranslational levels. PMID: 22993150
    23. The finding that patients with homozygous SLC2A2 mutations can have neonatal diabetes supports a role for GLUT2 in the human beta cell. PMID: 22660720
    24. Homozygous mutations in GLUT2, which cause Fanconi-Bickel syndrome, can lead to very different clinical and biochemical findings that are not limited to mild proximal renal tubulopathy but can include significant hypercalciuria. PMID: 22865906
    25. report on two siblings with Fanconi-Bickel syndrome (FBS) and an unusually mild clinical course; both patients were found to be compound heterozygous for the novel GLUT2 (SLC2A2) mutations c.457_462delCTTATA (p.153_4delLI) and c.1250C>G (p.P417R) PMID: 22214819
    26. Constitutive expression of GLUT2 in the apical membrane along with additional translocation of cytoplasmic GLUT2 to the apical membrane via an intact cytoskeleton and activated PKC appears responsible for enhanced carrier-mediated glucose uptake. PMID: 21943636
    27. We report the first Chinese cases of Fanconi-Bickel syndrome (FBS),a rare inherited disease caused by mutations in the glucose transporter 2 gene, SLC2A2. PMID: 22145468
    28. In human enterocytes, GLUT2 was consistently located in basolateral membranes; mice on a low-carbohydrate/high-fat diet for 12 months also exhibited endosomal GLUT2 accumulation and reduced glucose absorption. PMID: 21852673
    29. Polyphenols, phenolic acids and tannins from strawberry and apple are potent inhibitors of GLUT2 and SGLT1 at concentrations predicted after dietary ingestion. PMID: 20564476
    30. prostate cancer was inversely associated with the SLC2A2 rs5400 Thr110 allele PMID: 20142250
    31. Intestinal glucose absorption by the apical GLUT2 pathway can be 3 to 5-times greater then by SGLT1 et the high concentration of sugar. PMID: 20201351
    32. Genetic polymorphisms of SLC2A2 and HP is associated with serum cholesterol levels. PMID: 20066028
    33. mutated in patients with fanconi-Bickel syndrome PMID: 11810292
    34. Hepatocyte nuclear factor-1alpha recruits the transcriptional co-activator p300 on the GLUT2 gene promoter. PMID: 11978637
    35. polymorphisms at positions -269, -44, or + 103 may affect GLUT2 gene transcription, possibly associated with reduced expression of the GLUT2 gene in NIDDM patients. PMID: 12017192
    36. Expression is responsible for resistance to alloxan and streptozotocin toxicity. PMID: 14614558
    37. We have found GLUT-2 and glucokinase mRNAs in several brain regions, including the ventromedial and arcuate nuclei of the hypothalamus PMID: 15009676
    38. SNPs of SLC2A2 predict the conversion to diabetes in obese subjects with impaired glucose tolerance. PMID: 15983230
    39. identify Glut2 as a GroPIns transporter in mammals, and define a physiologically relevant cell-permeation mechanism PMID: 17141226
    40. kidney of diabetic rats, an initial and transient upregulation of GLUT2 was induced specifically by insulin only. PMID: 17204838
    41. recent progress in elucidating the transcriptional regulation of GLUT2 in the liver and pancreatic beta-cells and the relevance to type 2 diabetes.[RREVIEW] PMID: 18220613
    42. Data show that glucose transport in human airway epithelial cells in vitro and in vivo utilises GLUT2 transporters, and suggest that these transporters could contribute to glucose uptake/homeostasis in the human airway. PMID: 18239936
    43. Our findings show that a genetic variation in GLUT2 is associated with habitual consumption of sugars, suggesting an underlying glucose-sensing mechanism that regulates food intake. PMID: 18349384
    44. The contribution of GLUT2 to human metabolic diseases (Review) PMID: 19223655
    45. combined presence of rs5393 & rs5394 polymorphisms of GLUT2 was more frequent in type 2 diabetics than non-diabeteics; rs5394 appeared to be associated with decreased glucose stimulated insulin release & a tendency to a reduced GLUT2 gene expression PMID: 19269875
    46. The expression pattern of GLUT2 is reported in newly diagnosed esophageal adenocarcinoma by means of immunohistochemistry. PMID: 19554504

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  • 相关疾病：
    Fanconi-Bickel syndrome (FBS)
  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Major facilitator superfamily, Sugar transporter (TC 2.A.1.1) family, Glucose transporter subfamily
  • 组织特异性：
    Liver, insulin-producing beta cell, small intestine and kidney.
  • 数据库链接：

    HGNC: 11006

    OMIM: 138160

    KEGG: hsa:6514

    STRING: 9606.ENSP00000323568

    UniGene: Hs.167584

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