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  4. PKN2 Recombinant Monoclonal Antibody

PKN2 Recombinant Monoclonal Antibody

说明书
  • 中文名称：
    PKN2重组抗体
  • 货号：
    CSB-RA056240A0HU
  • 规格：
    ￥1320
  • 图片：
    • Western Blot
      Positive WB detected in: Hela whole cell lysate, 293T whole cell lysate
      All lanes: EBI3 antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 140 kDa
      Observed band size: 140 kDa
    • Overlay Peak curve showing HepG2 cells stained with CSB-RA056240A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

产品详情

  • 产品描述：
    CSB-RA056240A0HU PKN2重组单克隆抗体是针对蛋白激酶N2（PKN2）研发的高特异性科研试剂。该抗体经多种实验验证，在Western Blot中可识别天然及过表达的PKN2蛋白，在流式细胞术中能有效检测细胞内源性表达，推荐使用稀释度分别为1:500-1:2000和1:50-1:200。PKN2作为Rho效应激酶，参与调控细胞骨架重组、细胞极性建立及肿瘤细胞迁移等关键生物学过程，其异常表达与多种病理机制相关。本产品采用重组单克隆技术制备，具有高亲和力与优异的批间稳定性，适用于探索PKN2在细胞信号转导、肿瘤微环境调控及代谢性疾病中的分子机制研究，特别推荐用于蛋白质互作分析、亚细胞定位观察及功能缺失/获得性实验中的靶标验证，为细胞生物学与转化医学研究提供可靠工具。
  • Uniprot No.：
    Q16513
  • 基因名：
    PKN2
  • 别名：
    Serine/threonine-protein kinase N2 (EC 2.7.11.13) (PKN gamma) (Protein kinase C-like 2) (Protein-kinase C-related kinase 2), PKN2, PRK2 PRKCL2
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from Human PKN2
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    11H3
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, FC
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:2000
    FC 1:50-1:200
  • Protocols：
    ELISA Protocol
    Western Blotting (WB) Protocol
    Flow cytometry (FC) Protocol
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

产品评价

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PKN2 antibodies

PKN2 Proteins

靶点详情

  • 功能：
    PKC-related serine/threonine-protein kinase and Rho/Rac effector protein that participates in specific signal transduction responses in the cell. Plays a role in the regulation of cell cycle progression, actin cytoskeleton assembly, cell migration, cell adhesion, tumor cell invasion and transcription activation signaling processes. Phosphorylates CTTN in hyaluronan-induced astrocytes and hence decreases CTTN ability to associate with filamentous actin. Phosphorylates HDAC5, therefore lead to impair HDAC5 import. Direct RhoA target required for the regulation of the maturation of primordial junctions into apical junction formation in bronchial epithelial cells. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Stimulates FYN kinase activity that is required for establishment of skin cell-cell adhesion during keratinocytes differentiation. Regulates epithelial bladder cells speed and direction of movement during cell migration and tumor cell invasion. Inhibits Akt pro-survival-induced kinase activity. Mediates Rho protein-induced transcriptional activation via the c-fos serum response factor (SRF). Involved in the negative regulation of ciliogenesis.; (Microbial infection) Phosphorylates HCV NS5B leading to stimulation of HCV RNA replication.
  • 基因功能参考文献：
    1. The expression of PKN2 in colon cancer cells suppresses tumor associated M2 macrophage polarization and tumor growth. PMID: 29368606
    2. PKN2 knockdown in vitro decreased insulin-stimulated glucose uptake, incorporation into glycogen, and oxidation. Thus, PKN2 alters key signaling pathways and transcriptional networks to regulate glucose and lipid metabolism. PMID: 28720584
    3. Helicobacter pylori CagA interacts with PRK2 and inhibits its kinase activity. PMID: 26041307
    4. findings demonstrate that Yersinia enterocolitica rYopM interacts with RSK1 and PRK2 following cell-penetration PMID: 25513777
    5. Regulation of protein kinase C-related protein kinase 2 (PRK2) by an intermolecular PRK2-PRK2 interaction mediated by Its N-terminal domain. PMID: 22511787
    6. these findings suggest that Hsp90 plays a critical role in the regulation of HCV RNA polymerase phosphorylation via the PDK1-PRK2 signaling pathway. PMID: 22490666
    7. Rho binding is essential for PRK2 function and facilitates PRK2 recruitment to junctions. Kinase-dead PRK2 acts as a dominant-negative mutant and prevents apical junction formation. PMID: 20974804
    8. PRK2/PKN2, is an essential regulator of both entry into mitosis and exit from cytokinesis in HeLa S3 cells, required for abscission of the midbody at the end of the cell division cycle and for phosphorylation and activation of Cdc25B. PMID: 17332740
    9. These observations highlight elements of Nef's functional complexity and demonstrate previously unsuspected structural requirements for PAK-2 activation and MHC-1 down-modulation in Nef's flexible N- and C-terminal regions. PMID: 17632197
    10. Little evidence of association was observed between SNPs in PKN2 and type 2 diabetes in African Americans. PMID: 18443202
    11. the last seven amino acid residues at the C-terminus of PRK2 are not required for the activation of the kinase by RhoA in vitro, however, the extreme C-terminal segment is critical for the full activation of PRK2 by RhoA in cells PMID: 18835241

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  • 亚细胞定位：
    Cytoplasm. Nucleus. Membrane. Cell projection, lamellipodium. Cytoplasm, cytoskeleton. Cleavage furrow. Midbody. Cell junction.
  • 蛋白家族：
    Protein kinase superfamily, AGC Ser/Thr protein kinase family, PKC subfamily
  • 组织特异性：
    Ubiquitous. Expressed in numerous tumor cell lines, especially in bladder tumor cells.
  • 数据库链接：

    HGNC: 9406

    OMIM: 602549

    KEGG: hsa:5586

    STRING: 9606.ENSP00000359552

    UniGene: Hs.440833

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