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  4. OS9 Recombinant Monoclonal Antibody

OS9 Recombinant Monoclonal Antibody

说明书
  • 中文名称：
    OS9重组抗体
  • 货号：
    CSB-RA959292A0HU
  • 规格：
    ￥1320
  • 图片：
    • Western Blot
      Positive WB detected in: HL-60 whole cell lysate, Hela whole cell lysate, Mouse kidney tissue
      All lanes: OS9 antibody at 1:2000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 76, 70, 68, 74, 66, 64, 70, 61 kDa
      Observed band size: 72-95 kDa
    • IHC image of CSB-RA959292A0HU diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
  • 其他：

产品详情

  • 产品描述：
    CSB-RA959292A0HU OS9重组单克隆抗体是针对内质网相关降解通路关键调控因子OS9蛋白开发的高特异性科研工具。该抗体识别的OS9蛋白在细胞内负责介导错误折叠蛋白的识别与降解调控，其异常表达与多种细胞应激反应及病理过程密切相关。经多平台验证显示，该抗体在Western Blot实验中可清晰检测目标蛋白表达，在免疫组织化学应用中能有效显示组织切片中OS9的亚细胞定位特征，同时在ELISA体系中也表现出优异的抗原结合能力。推荐使用稀释度为WB 1:500-1:2000、IHC 1:50-1:200，实验数据显示其在不同样本类型中均保持稳定的检测灵敏度和低背景干扰。该重组单克隆抗体采用哺乳动物表达系统制备，具有高度均一的批次稳定性，适用于研究蛋白质质量控制机制、内质网应激相关信号通路以及肿瘤等疾病模型中异常蛋白代谢的分子机制，为探索细胞稳态调控及疾病相关分子网络提供可靠的研究工具。
  • Uniprot No.：
    Q13438
  • 基因名：
    OS9
  • 别名：
    Protein OS-9 (Amplified in osteosarcoma 9), OS9
  • 反应种属：
    Human, Mouse
  • 免疫原：
    A synthesized peptide derived from human OS9
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    26H3
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:2000
    IHC 1:50-1:200
  • Protocols：
    ELISA Protocol
    Western Blotting (WB) Protocol
    Immunohistochemistry (IHC) Protocol
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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OS9 antibodies

OS9 Proteins

靶点详情

  • 功能：
    Lectin which functions in endoplasmic reticulum (ER) quality control and ER-associated degradation (ERAD). May bind terminally misfolded non-glycosylated proteins as well as improperly folded glycoproteins, retain them in the ER, and possibly transfer them to the ubiquitination machinery and promote their degradation. Possible targets include TRPV4.
  • 基因功能参考文献：
    1. The endoplasmic reticulum-associated protein, OS-9, behaves as a lectin in targeting the immature calcium-sensing receptor. PMID: 28419469
    2. OS-9 up-regulates occludin and claudin-1 by activating the MAP kinase (MAPK) pathway, and thus protects the epithelial barrier function of Caco-2 monolayer under hypoxia condition. PMID: 25999789
    3. EDEM2 and OS-9 are required for ER-associated degradation of non-glycosylated sonic hedgehog PMID: 24910992
    4. Unique regions mediate the interaction between OS-9 and GRP94. PMID: 24899641
    5. It delivers mutant neuroserpin to ERAD by recognition of glycan side chains and provide the first in vivo proof of involvement of ERAD in degradation of mutant neuroserpin. PMID: 24795221
    6. long non-coding RNA ENST00000480739 suppresses tumour cell invasion by regulating OS-9 and HIF-1alpha in pancreatic ductal adenocarcinoma. PMID: 25314054
    7. Data indicate that the interaction of OS-9 and XTP3-B with CD147(CG) was inhibited by mutations to conserved residues in their lectin domains. PMID: 23097496
    8. OS-9 plays no direct functional role in HIF degradation since physical interaction of OS-9 with oxygen sensing HIF prolyl hydroxylases cannot occur in vivo due to their different subcellular localization PMID: 21559462
    9. It regulates endoplasmic reticulum-associated degradation. (review) PMID: 21404621
    10. The OS-9 specifically recognizes Manalpha1,6Manalpha1,6Man residues on the processed C-arm through the continuous double tryptophan (WW) motif. PMID: 21172656
    11. The sugar-binding ability of human OS-9 and its involvement in ER-associated degradation PMID: 19914915
    12. Identification of a peptide, resulting from a point mutation in gene OS-9, recognized by cytolytic T lymphocytes on a human melanoma. PMID: 12747754
    13. OS-9 regulates the secretory transport of TRPV4 and appears to protect TRPV4 subunits from the precocious ubiquitination and ER-associated degradation. PMID: 17932042
    14. OS-9 and GRP94 deliver mutant alpha1-antitrypsin to the Hrd1-SEL1L ubiquitin ligase complex for ERAD PMID: 18264092
    15. Xbp1-responsive OS-9 variants in the mammalian endoplasmic reticulum inhibit secretion of misfolded protein conformers and enhancing their disposal PMID: 18417469
    16. OS9 is critically involved in the modulation of ER-to-Golgi transport of DC-STAMP in response to TLR triggering, suggesting a novel role for OS9 in myeloid differentiation and cell fusion. PMID: 18952287
    17. OS-9 recognises terminally misfolded proteins via polypeptide-based rather than glycan-based signals, but is only required for transferring those bearing N-glycans to the ubiquitination machinery. PMID: 19084021
    18. model for mannose trimming and the requirement for OS-9 lectin activity in glycoprotein ERAD in which N-glycans lacking the terminal mannose from the C branch are recognized by OS-9 and targeted for degradation PMID: 19346256

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  • 亚细胞定位：
    Endoplasmic reticulum lumen.
  • 蛋白家族：
    OS-9 family
  • 组织特异性：
    Ubiquitously expressed. Found as well in all tumor cell lines analyzed, amplified in sarcomas. Highly expressed in osteosarcoma SJSA-1 and rhabdomyosarcoma Rh30 cell lines. Isoform 2 is the major isoform detected in all cell types examined.
  • 数据库链接：

    HGNC: 16994

    OMIM: 609677

    KEGG: hsa:10956

    STRING: 9606.ENSP00000318165

    UniGene: Hs.527861

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