MAPK1/MAPK3 Recombinant Monoclonal Antibody

中文名称：

MAPK1/MAPK3重组抗体
货号：

CSB-RA984568A0HU
规格：

￥1320
图片：
Western Blot
Positive WB detected in: THP-1 whole cell lysate, HEPG2 whole cell lysate,MCF7 whole cell lysate,293 whole cell lysate,K562 whole cell lysate,PC-3 whole cell lysate, Mouse brain tissue lysate
All lanes: ERK1/2 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 42,44 kDa
Observed band size: 42,44 kDa

IHC image of CSB-RA984568A0HU diluted at 1:50 and staining in paraffin-embedded human stomach tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.64% DAB.

Overlay Peak curve showing Hela cells surface stained with CSB-RA984568A0HU (red line) at 1:50. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1µg/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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产品描述：

MAPK1/MAPK3重组单克隆抗体（CSB-RA984568A0HU）靶向丝裂原活化蛋白激酶ERK1/ERK2，该家族蛋白在细胞增殖、分化及应激响应等关键信号通路中发挥核心调控作用。本抗体经多种实验验证，在Western Blot中可特异性识别内源性靶蛋白，免疫组化实验显示其在多种组织样本中具有清晰的亚细胞定位，流式细胞术进一步证实其适用于活细胞表面检测，推荐稀释度分别为WB 1:500-1:2000、IHC 1:50-1:200、FC 1:50-1:200，同时支持ELISA平台的高灵敏度检测。该产品通过严格的抗原表位筛选和重组表达技术制备，具备高批次一致性和低交叉反应性，适用于肿瘤发生机制、神经信号传导、细胞周期调控等领域的基础研究，尤其为MAPK/ERK通路相关药物靶点探索及疾病模型构建提供可靠工具。本试剂仅限科研用途，需配合标准实验条件使用。
Uniprot No.：

P27361 P28482
基因名：

MAPK1/MAPK3
别名：

Mitogen-activated protein kinase 3 (MAP kinase 3) (MAPK 3) (EC 2.7.11.24) (ERT2) (Extracellular signal-regulated kinase 1) (ERK-1) (Insulin-stimulated MAP2 kinase) (MAP kinase isoform p44) (p44-MAPK) (Microtubule-associated protein 2 kinase) (p44-ERK1), MAPK3, ERK1 PRKM3; Mitogen-activated protein kinase 1 (MAP kinase 1) (MAPK 1) (EC 2.7.11.24) (ERT1) (Extracellular signal-regulated kinase 2) (ERK-2) (MAP kinase isoform p42) (p42-MAPK) (Mitogen-activated protein kinase 2) (MAP kinase 2) (MAPK 2), MAPK1, ERK2 PRKM1 PRKM2
反应种属：

Human, Mouse
免疫原：

A synthesized peptide derived from Human MAPK1/MAPK3
免疫原种属：

Homo sapiens (Human)
标记方式：

Non-conjugated
克隆类型：

Monoclonal
抗体亚型：

Rabbit IgG
纯化方式：

Affinity-chromatography
克隆号：

29B10
浓度：

It differs from different batches. Please contact us to confirm it.
保存缓冲液：

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
产品提供形式：

Liquid
应用范围：

ELISA, WB, IHC, FC
推荐稀释比：

Application Recommended Dilution

WB 1:500-1:2000

IHC 1:50-1:200

FC 1:50-1:200
Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Flow cytometry (FC) Protocol
储存条件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
货期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

MAPK1/MAPK3 Recombinant Monoclonal Antibody

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Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
FC	1:50-1:200