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  4. MADCAM1 Recombinant Monoclonal Antibody

MADCAM1 Recombinant Monoclonal Antibody

说明书
  • 中文名称：
    MADCAM1重组抗体
  • 货号：
    CSB-RA215817A0HU
  • 规格：
    ￥1320
  • 图片：
    • Overlay Peak curve showing PC3 cells surface stained with CSB-RA215817A0HU (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

产品详情

  • 产品描述：
    CSB-RA215817A0HU MADCAM1重组单克隆抗体是针对黏膜地址素细胞黏附分子1（MADCAM1）开发的高特异性科研试剂。MADCAM1是一种主要表达于血管内皮细胞的跨膜蛋白，尤其在肠道相关淋巴组织中高表达，通过结合α4β7整合素和L-选择素介导淋巴细胞向黏膜组织的定向迁移，在免疫监视和炎症反应中发挥关键调控作用。本产品经ELISA和流式细胞术（FC）严格验证，可实现对天然及重组MADCAM1蛋白的特异性识别，其中流式检测推荐使用稀释比例为1:50-1:200。该抗体适用于体外研究MADCAM1在免疫细胞归巢、黏膜屏障免疫调节及慢性炎症疾病机制中的功能，例如通过流式细胞术分析内皮细胞表面MADCAM1的表达动态，或结合体外黏附实验探究淋巴细胞与血管内皮相互作用的分子基础。作为重组单克隆抗体，其具有批次稳定性和高亲和力优势，可为免疫学、炎症相关疾病研究提供可靠工具。
  • Uniprot No.：
    Q13477
  • 基因名：
    MADCAM1
  • 别名：
    Mucosal addressin cell adhesion molecule 1 (MAdCAM-1) (hMAdCAM-1), MADCAM1
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from human MADCAM1
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    12B5
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, FC
  • 推荐稀释比：
    Application Recommended Dilution
    FC 1:50-1:200
  • Protocols：
    ELISA Protocol
    Flow cytometry (FC) Protocol
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

产品评价

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MADCAM1 antibodies

MADCAM1 Proteins

靶点详情

  • 功能：
    Cell adhesion leukocyte receptor expressed by mucosal venules, helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes. Isoform 2, lacking the mucin-like domain, may be specialized in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independent of L-selectin binding.
  • 基因功能参考文献：
    1. Both the ACT-1 assay and the MAdCAM-1 assay demonstrated acceptable reproducibility and repeatability. The assays were sufficiently stable to allow for clinical use. During clinical testing the assays demonstrated that vedolizumab was able to saturate peripheral cells at all doses tested. PMID: 25908521
    2. Doxo-induced apoptosis has a role in inhibiting hepatocellular carcinoma by targeting Madcam1 and AKT and blocking protein translation initiation PMID: 26124182
    3. MADCAM1 is up-regulated in liver cirrhosis with expression on peribiliary vascular plexus and lymphoid aggregates PMID: 23839340
    4. Domain 1 of mucosal addressin cell adhesion molecule has an I1-set fold and a flexible integrin-binding loop. PMID: 23297416
    5. Findings suggest that the inactive alpha4beta7 and alpha4beta7 activated by different stimuli have distinct conformations with different structural requirements for MAdCAM-1 binding. PMID: 21296888
    6. Ca2+ is essential for cell tethering in flow and rolling interactions through alpha 4 integrins with MAdCAM-1 as substrate; Mg2+ promotes firm adhesion of cells on MAdCAM-1 but with much lower tethering efficiency in shear flow. PMID: 11705388
    7. A crystal structure for the two extracellular amino-terminal domains of human MAdCAM-1 PMID: 11807247
    8. more extensive expression of MAdCAM-1 in Crohn's disease (CrD), than in ulcerative colitis, which could contribute not only to mucosal inflammation, but also to transmural inflammation in CrD. PMID: 12100519
    9. MAdCAM-1/integrin alpha4beta7 homing system may participate in gastric inflammation in response to H pylori-infection and contributes to MALT formation, typically leading to the development of nodular gastritis. PMID: 14669317
    10. a marker of graft rejection in small intestine transplantation PMID: 15050155
    11. PI3-K/Akt is involved in the gut-specific differentiation of human intestinal microvascular endothelial cells, which results in expression of the mucosal addressin MAdCAM-1 PMID: 15483224
    12. Immobilized CCL25 and CCL28 are both able to trigger integrin alpha(4)beta(7)-dependent lymphocyte arrest on MAdCAM-1 under shear flow. PMID: 18308860
    13. mucosal addressin cell adhesion molecule 1 may represent an important determinant for the generation of mucosal damage in celiac disease PMID: 19157500
    14. MADCAM1 gene polymorphisms may be associated with the risk of chronic GVHD and may, also, affect mortality related to aHSCT. PMID: 19286444

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  • 亚细胞定位：
    Membrane; Single-pass type I membrane protein.
  • 组织特异性：
    Highly expressed on high endothelial venules (HEV) and lamina propia venules found in the small intestine, and to a lesser extent in the colon and spleen. Very low levels of expression found in pancreas and brain. Not expressed in the thymus, prostate, ov
  • 数据库链接：

    HGNC: 6765

    OMIM: 102670

    KEGG: hsa:8174

    STRING: 9606.ENSP00000215637

    UniGene: Hs.102598

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