Your Good Partner in Biology Research

中文简体

CSF2｜ CTLA4｜ DLL3｜ EGFR｜ IL4｜ IL4R｜ LAG3｜ MSTN｜ NECTIN4｜ NT5E｜ SEZ6｜ TM4SF1｜ TNFSF13｜ TPO

  1. Home
  2. 抗体
  3. 重组抗体
  4. KIFAP3 Recombinant Monoclonal Antibody

KIFAP3 Recombinant Monoclonal Antibody

说明书
  • 中文名称：
    KIFAP3重组抗体
  • 货号：
    CSB-RA869779A0HU
  • 规格：
    ￥1320
  • 图片：
    • Western Blot
      Positive WB detected in: Hela whole cell lysate
      All lanes: KIFAP3 antibody at 1:2000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 92, 87, 83 kDa
      Observed band size: 72-95 kDa
    • IHC image of CSB-RA869779A0HU diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • Immunofluorescence staining of PC-3 cell with CSB-RA869779A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 552-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品描述：
    CSB-RA869779A0HU KIFAP3重组单克隆抗体是针对驱动蛋白家族成员KIFAP3研发的高特异性科研工具，适用于多种实验场景。该抗体识别的靶标蛋白KIFAP3在细胞内运输、有丝分裂调控及纤毛形成中发挥重要作用，其异常表达与肿瘤发生、神经退行性疾病密切相关。经严格验证，该抗体在Western Blot中可清晰检测目标蛋白，免疫组化（IHC）和免疫荧光（IF）实验显示其在多种组织与细胞模型中的特异性定位，推荐使用稀释度为WB 1:500-1:2000、IHC 1:50-1:200、IF 1:50-1:200，同时支持ELISA检测。其高批次间一致性为细胞周期研究、胞内运输机制探索及疾病相关分子网络解析提供可靠支持，适用于肿瘤生物学、神经科学领域的体外实验及病理模型分析，为研究KIFAP3在细胞动力学异常或病理微环境中的功能调控提供精准检测方案。
  • Uniprot No.：
    Q92845
  • 基因名：
    KIFAP3
  • 别名：
    Kinesin-associated protein 3 (KAP-3) (KAP3) (Smg GDS-associated protein), KIFAP3, KIF3AP SMAP
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from human KIFAP3
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    28E9
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:2000
    IHC 1:50-1:200
    IF 1:50-1:200
  • Protocols：
    ELISA Protocol
    Western Blotting (WB) Protocol
    Immunohistochemistry (IHC) Protocol
    Immunofluorescence (IF) Protocol
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

产品评价

相关产品

KIFAP3 antibodies

KIFAP3 Proteins

靶点详情

  • 功能：
    Involved in tethering the chromosomes to the spindle pole and in chromosome movement. Binds to the tail domain of the KIF3A/KIF3B heterodimer to form a heterotrimeric KIF3 complex and may regulate the membrane binding of this complex.
  • 基因功能参考文献：
    1. This study does not show an influence of KIFAP3 variants on survival in the studied Swiss and Swedish cohort PMID: 28140676
    2. Common genetic variation in the Kinesin-Associated Protein 3 (KIFAP3) gene (rs1541160) was reported to have a significant effect on survival in amyotrophic lateral sclerosis patients. PMID: 24838185
    3. The incidence of the motor neuron-predominant amyotrophic lateral sclerosis phenotype in the patients of this cohort supports the hypothesis that the SNP within the KIFAP3 gene is a potential modifier of the amyotrophic lateral sclerosis phenotype PMID: 21659726
    4. Data clearly show that SNP rs1541160 does not modify survival within cohort of 504 Italian ALS cases. PMID: 20566859
    5. Kinesin-2 plays a role in the KDEL-R-/COPI-dependent retrograde transport pathway from the Golgi complex to the ER. PMID: 17113389
    6. KAP3 sequestration by misfolded SOD1 and the inhibition of ChAT transport play a role in the dysfunction of amyotrophic lateral sclerosis. PMID: 19088126
    7. a variant within the KIFAP3 gene that is associated with decreased KIFAP3 expression and increased survival in sporadic ALS was identified. PMID: 19451621

    显示更多

    收起更多

  • 数据库链接：

    HGNC: 17060

    OMIM: 601836

    KEGG: hsa:22920

    STRING: 9606.ENSP00000354560

    UniGene: Hs.433442

Most popular with customers

产品中心
特别关注


X
九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |