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KDM1B Recombinant Monoclonal Antibody

说明书
  • 中文名称：
    KDM1B重组抗体
  • 货号：
    CSB-RA958367A0HU
  • 规格：
    ￥1320
  • 图片：
    • Western Blot
      Positive WB detected in: A549 whole cell lysate, Hela whole cell lysate
      All lanes: KDM1B antibody at 1:2000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 93, 66, 20 kDa
      Observed band size: 90-95, 17-28 kDa
  • 其他：

产品详情

  • 产品描述：
    KDM1B Recombinant Monoclonal Antibody（CSB-RA958367A0HU）是针对组蛋白修饰酶KDM1B（赖氨酸特异性去甲基酶1B）开发的高特异性科研工具，适用于表观遗传调控机制研究。该抗体通过重组技术制备，具有优异的批次一致性和稳定性，经ELISA和Western Blot（WB）严格验证，在1:500-1:2000稀释范围内可清晰检测内源性KDM1B蛋白，特异性经重组蛋白对照实验确认。KDM1B作为H3K4me1/2去甲基化酶，在胚胎发育、干细胞多能性维持及肿瘤发生中发挥重要调控作用。本产品适用于检测哺乳动物细胞裂解液或组织样本中KDM1B的表达水平，可支持表观遗传学、细胞分化、肿瘤生物学等领域的机制研究，尤其在探索基因沉默、染色质重塑及异常表观遗传修饰相关疾病模型中具有应用价值。实验建议配合阳性对照验证样本处理条件，避免交叉反应干扰。
  • Uniprot No.：
    Q8NB78
  • 基因名：
    KDM1B
  • 别名：
    Lysine-specific histone demethylase 1B (EC 1.-.-.-) (Flavin-containing amine oxidase domain-containing protein 1) (Lysine-specific histone demethylase 2), KDM1B, AOF1 C6orf193 LSD2
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from human KDM1B
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    15F3
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:2000
  • Protocols：
    ELISA Protocol
    Western Blotting (WB) Protocol
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

产品评价

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KDM1B antibodies

KDM1B Proteins

靶点详情

  • 功能：
    Histone demethylase that demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. Required for de novo DNA methylation of a subset of imprinted genes during oogenesis. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Demethylates both mono- and di-methylated 'Lys-4' of histone H3. Has no effect on tri-methylated 'Lys-4', mono-, di- or tri-methylated 'Lys-9', mono-, di- or tri-methylated 'Lys-27', mono-, di- or tri-methylated 'Lys-36' of histone H3, or on mono-, di- or tri-methylated 'Lys-20' of histone H4.
  • 基因功能参考文献：
    1. These results suggest that LSD2 achieves a promoting effect on small cell lung cancer by indirectly regulating TFPI2 expression through the mediation of DNMT3B expression or through the regulation of the demethylation of H3K4me1 in the promoter region of the TFPI2 gene. PMID: 29845195
    2. This review focuses on published small-molecule inhibitors targeted at the two flavin adenine dinucleotide-dependent lysine demethylases, lysine-specific demethylases 1 and 2, and how the inhibitors interact with the tertiary structures of the enzymes. PMID: 28277979
    3. These results demonstrate an important role for LSD2 in regulation of DNA methylation and gene silencing in breast cancer. PMID: 24924415
    4. Histone demethylase LSD2 acts as an E3 ubiquitin ligase and inhibits cancer cell growth through promoting proteasomal degradation of OGT. PMID: 25773598
    5. Using transcriptome and chromatin immunoprecipitation-sequencing analyses, the study revealed that LSD2 represses the genes involved in lipid influx and metabolism through demethylation of histone H3K4. PMID: 25624347
    6. regulation of tissue factor pathway inhibitor-2 (TFPI-2) expression by lysine-specific demethylase 1 and 2 PMID: 25036127
    7. The zinc finger-SWIRM-oxidase domains is required for KDM1B demethylase activity and the binding of FAD. PMID: 23266887
    8. Human LSD2/KDM1b/AOF1 regulates gene transcription by modulating intragenic H3K4me2 methylation. PMID: 20670891

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    Flavin monoamine oxidase family
  • 数据库链接：

    HGNC: 21577

    OMIM: 613081

    KEGG: hsa:221656

    STRING: 9606.ENSP00000297792

    UniGene: Hs.709336

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