ILDR2 Recombinant Monoclonal Antibody

中文名称：

ILDR2 Recombinant Monoclonal Antibody
货号：

CSB-RA751251MA1HU
规格：

￥1320
图片：
Immunofluorescence staining of Hela cell with CSB-RA751251MA1HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Human IgG(H+L).

Immunofluorescence staining of U251 cell with CSB-RA751251MA1HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Human IgG(H+L).

Overlay Peak curve showing Jurkat cells stained with CSB-RA751251MA1HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100.Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-human IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was human IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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产品描述：

CSB-RA751251MA1HU ILDR2重组单克隆抗体是经ELISA、免疫荧光（IF）和流式细胞术（FC）严格验证的高特异性科研工具。该抗体靶向ILDR2蛋白，该分子作为免疫球蛋白超家族成员，在细胞间连接复合体形成及上皮极性建立中发挥重要作用，与组织屏障功能调控密切相关。实验数据显示，该抗体在IF应用中能清晰呈现细胞膜或特定亚细胞结构的定位信号，推荐使用稀释比例为1:50-1:200；在FC检测中可有效识别天然构象的靶蛋白，推荐同等稀释范围。经ELISA验证其具备优异的目标蛋白结合能力，适用于体外蛋白互作分析。本产品可广泛应用于细胞连接机制研究、上皮组织发育模型构建，以及相关信号通路的功能解析等基础研究领域，为探索ILDR2在细胞极性调控和生理病理过程中的分子机制提供可靠支持。
Uniprot No.：

Q71H61
基因名：

ILDR2
别名：

ILDR2; C1orf32Immunoglobulin-like domain-containing receptor 2
反应种属：

Human
免疫原：

Recombinant Human ILDR2 protein
免疫原种属：

Homo sapiens (Human)
标记方式：

Non-conjugated
克隆类型：

Monoclonal
抗体亚型：

hIgG1
纯化方式：

Affinity-chromatography
克隆号：

13G7
浓度：

It differs from different batches. Please contact us to confirm it.
保存缓冲液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
产品提供形式：

Liquid
应用范围：

ELISA, IF, FC
推荐稀释比：

Application Recommended Dilution

IF 1:50-1:200

FC 1:50-1:200
Protocols：

ELISA Protocol
Immunofluorescence (IF) Protocol
Flow cytometry (FC) Protocol
储存条件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
货期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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功能：

May be involved in lipid homeostasis and ER stress pathways.
基因功能参考文献：
1. We demonstrated that ZNF70 interacts with ZFP64 and activates HES1 transcription by binding to the HES1 promoter. In addition, HES1 gene expression is increased in ILDR2-knockdown HepG2 cells, in which ZNF70 is translocated from the cytoplasm to the nucleus, suggesting that ZNF70 migration to the nucleus after dissociating from the ILDR2-ZNF70 complex activates HES1 transcription. These results support a novel link betwee PMID: 27353377
2. Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator) PMID: 20379614
3. Observational study of gene-disease association. (HuGE Navigator) PMID: 19536175
4. Observational study of gene-disease association. (HuGE Navigator) PMID: 19064610
亚细胞定位：

Endoplasmic reticulum membrane; Single-pass type I membrane protein.
蛋白家族：

Immunoglobulin superfamily, LISCH7 family
数据库链接：

HGNC: 18131

KEGG: hsa:387597

UniGene: Hs.444835

ILDR2 Recombinant Monoclonal Antibody

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Application	Recommended Dilution
IF	1:50-1:200
FC	1:50-1:200