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  4. HMGCS2 Recombinant Monoclonal Antibody

HMGCS2 Recombinant Monoclonal Antibody

说明书
  • 中文名称：
    HMGCS2重组抗体
  • 货号：
    CSB-RA693900A0HU
  • 规格：
    ￥1320
  • 图片：
    • Western Blot
      Positive WB detected in: A549 whole cell lysate, Ntera-2 whole cell lysate
      All lanes: HMGCS2 antibody at 1:500
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 57, 53, 51 kDa
      Observed band size: 55 kDa
    • IHC image of CSB-RA693900A0HU diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • IHC image of CSB-RA693900A0HU diluted at 1:100 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
  • 其他：

产品详情

  • 产品描述：
    CSB-RA693900A0HU HMGCS2重组单克隆抗体是针对线粒体酶3-羟基-3-甲基戊二酰辅酶A合成酶2（HMGCS2）开发的高特异性科研工具。HMGCS2是酮体生成途径中的限速酶，参与调控肝脏能量代谢稳态，其表达异常与代谢性疾病、神经系统疾病及肿瘤发生相关。本抗体经ELISA、Western Blot（WB）和免疫组化（IHC）多平台验证，在WB实验中呈现清晰单一目标条带，推荐使用稀释度为1:500-1:2000；IHC应用显示优异的组织定位能力，建议稀释比为1:50-1:200。其重组单克隆特性确保了批次间的高度一致性，适用于检测人类样本中HMGCS2的蛋白表达水平及亚细胞定位分析。该产品可广泛应用于代谢重编程机制研究、肝脏或肾脏疾病模型中的能量代谢评估，以及肿瘤微环境与细胞代谢互作等基础研究领域，为探索HMGCS2在生理病理过程中的分子调控网络提供可靠检测工具。
  • Uniprot No.：
    P54868
  • 基因名：
    HMGCS2
  • 别名：
    Hydroxymethylglutaryl-CoA synthase, mitochondrial (HMG-CoA synthase) (EC 2.3.3.10) (3-hydroxy-3-methylglutaryl coenzyme A synthase), HMGCS2
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from human HMGCS2
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    19C11
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:2000
    IHC 1:50-1:200
  • Protocols：
    ELISA Protocol
    Western Blotting (WB) Protocol
    Immunohistochemistry (IHC) Protocol
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

产品评价

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HMGCS2 antibodies

HMGCS2 Proteins

靶点详情

  • 功能：
    Catalyzes the first irreversible step in ketogenesis, condensing acetyl-CoA to acetoacetyl-CoA to form HMG-CoA, which is converted by HMG-CoA reductase (HMGCR) into mevalonate.
  • 基因功能参考文献：
    1. several nutrients as well as specific nutritional related hormonal conditions are able to affect significantly HMGCS2 gene expression. PMID: 28888048
    2. miR-107-mediated decrease of HMGCS2 indicates poor outcomes and promotes cell migration in hepatocellular carcinoma PMID: 28867541
    3. this studies demonstrate that HMGCS2 functions as a transcriptional factor that binds to peroxisome proliferator-activated receptor alpha (PPARalpha), resulting in Src expression and activation in a metabolically independent manner PMID: 27816970
    4. HMGCS2 shares with ketone bodies common features in autophagic clearance of APP, suggesting that ketone bodies play an important role in HMGCS2 regulation of the autophagy. PMID: 28320515
    5. High expression of either HSD17B2 or HMGCS2 predicted poor susceptibility of rectal cancer to preoperative chemoradiotherapy. PMID: 25929810
    6. FABP7 and HMGCS2 may have roles in apocrine differentiation categorizing apocrine carcinoma of the breast PMID: 25389781
    7. In Fe-S cluster-deficient muscles, results show a dramatic up-regulation of the ketogenic enzyme HMGCS2 and the secreted protein FGF21 (fibroblast growth factor 21). PMID: 23943793
    8. Human HMGCS2 regulates mitochondrial fatty acid oxidation and FGF21 expression PMID: 21502324
    9. Ketogenesis is an undesirable metabolic characteristic of the proliferating cell, which is down-regulated through c-Myc-mediated repression of the key metabolic gene HMGCS2. PMID: 16940161

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  • 相关疾病：
    3-hydroxy-3-methylglutaryl-CoA synthase-2 deficiency (HMGCS2D)
  • 亚细胞定位：
    Mitochondrion.
  • 蛋白家族：
    HMG-CoA synthase family
  • 组织特异性：
    Expression in liver is 200-fold higher than in any other tissue. Low expression in colon, kidney, testis, and pancreas. Very low expression in heart and skeletal muscle. Not detected in brain.; [Isoform 3]: Highest expression detected in heart and skeleta
  • 数据库链接：

    HGNC: 5008

    OMIM: 600234

    KEGG: hsa:3158

    STRING: 9606.ENSP00000358414

    UniGene: Hs.59889

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