DNAJA1 Recombinant Monoclonal Antibody

中文名称：

DNAJA1重组抗体
货号：

CSB-RA589492A0HU
规格：

￥1320
图片：
Western Blot
Positive WB detected in: U251 whole cell lysate, Ntera-2 whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate, Hela whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate
All lanes: DNAJA1 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 45, 38 kDa
Observed band size: 45-55 kDa

Immunofluorescence staining of A549 cell with CSB-RA589492A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 550-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Overlay Peak curve showing A549 cells stained with CSB-RA589492A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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产品描述：

DNAJA1 Recombinant Monoclonal Antibody（CSB-RA589492A0HU）是一款经多种实验验证的高特异性抗体，靶向DNAJA1蛋白。DNAJA1是Hsp40/DnaJ分子伴侣家族成员，通过调节Hsp70 ATP酶活性参与蛋白质折叠、应激反应及错误蛋白降解，其表达异常与肿瘤发生、神经退行性疾病等病理过程密切相关。本抗体经ELISA、Western Blot（WB）、免疫荧光（IF）和流式细胞术（FC）严格验证，在WB实验中可清晰识别内源性DNAJA1蛋白，IF与FC实验能精准定位细胞内及膜表面靶蛋白分布，推荐稀释度分别为WB 1:500-1:2000、IF 1:50-1:200、FC 1:50-1:200。验证数据表明该抗体在多种样本类型中均呈现低背景和高信噪比特性，适用于研究DNAJA1在细胞应激应答、蛋白质质量控制机制中的功能，或用于体外模型中探索其与Hsp70协同作用对疾病相关蛋白聚集的调控。该产品为研究分子伴侣网络、疾病相关蛋白质稳态失衡提供可靠工具，适用于基础科研领域的蛋白互作分析、亚细胞定位研究及功能验证实验。
Uniprot No.：

P31689
基因名：

DNAJA1
别名：

DnaJ homolog subfamily A member 1 (DnaJ protein homolog 2) (HSDJ) (Heat shock 40 kDa protein 4) (Heat shock protein J2) (HSJ-2) (Human DnaJ protein 2) (hDj-2), DNAJA1, DNAJ2 HDJ2 HSJ2 HSPF4
反应种属：

Human
免疫原：

A synthesized peptide derived from human DNAJA1
免疫原种属：

Homo sapiens (Human)
标记方式：

Non-conjugated
克隆类型：

Monoclonal
抗体亚型：

Rabbit IgG
纯化方式：

Affinity-chromatography
克隆号：

23C6
浓度：

It differs from different batches. Please contact us to confirm it.
保存缓冲液：

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
产品提供形式：

Liquid
应用范围：

ELISA, WB, IF, FC
推荐稀释比：

Application Recommended Dilution

WB 1:500-1:2000

IF 1:50-1:200

FC 1:50-1:200
Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunofluorescence (IF) Protocol
Flow cytometry (FC) Protocol
储存条件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
货期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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功能：

Co-chaperone for HSPA8/Hsc70. Stimulates ATP hydrolysis, but not the folding of unfolded proteins mediated by HSPA1A (in vitro). Plays a role in protein transport into mitochondria via its role as co-chaperone. Functions as co-chaperone for HSPA1B and negatively regulates the translocation of BAX from the cytosol to mitochondria in response to cellular stress, thereby protecting cells against apoptosis. Promotes apoptosis in response to cellular stress mediated by exposure to anisomycin or UV.
基因功能参考文献：
1. that DNAJA1 controls the fate of misfolded stabilization of mutant p53 PMID: 27775703
2. Therefore, we propose that DnaJA1 is co-opted by the influenza A virus to enter the nucleus and to enhance its RNA polymerase activity in an Hsp70 cochaperone-independent manner. PMID: 25253355
3. structure and function of human DNAJA1 and its relationship to pancreatic cancer PMID: 24512202
4. we combined the Hsp70-NEF pairs with cochaperones of the J protein family (DnaJA1, DnaJA2, DnaJB1, and DnaJB4) to generate 16 permutations. PMID: 24318877
5. Moreover, the levels of DnaJA1 and Hsp70 seem to play against each other with regard to tau: as DnaJA1 levels increase, tau levels are reduced, but this can be prevented if Hsp70 levels are simultaneously induced. PMID: 22343013
6. Hdj2 directly associates with Japanese encephalitis virus nonstructural protein NS5 and facilitates viral replication. PMID: 21999493
7. Hsc70 and a dimer of DjA1 independently bind to an unfolded protein PMID: 20363747
8. Hsp40 type 1 chaperones DJA1 (DNAJA1/Hdj2) and DJA2 (DNAJA2) as key modulators of hERG degradation PMID: 19940115
9. Mammalian, yeast, bacterial, and chemical chaperones reduce aggregate formation and death in a cell model of oculopharyngeal muscular dystrophy PMID: 11796717
10. HSP40 binding is the first step in the HSP90 chaperoning pathway for the progesterone receptor PMID: 11809754
11. sequence analysis of two isoforms PMID: 12974469
12. Multiple tissue polymerase chain reaction (PCR) results showed that nDnaJA1 expressed highly in testis and lung but low in thymus, prostate, colon and liver PMID: 15595953
13. Results indicate that DjA1 and DjB4 of subfamilies A and B of human Hsp40 have different quaternary structures and chaperone functions. PMID: 15661747
14. DJA1 was inhibitory of refolding with DJA2 and Hsc70. PMID: 18684711
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亚细胞定位：

Membrane; Lipid-anchor. Cytoplasm. Microsome. Nucleus. Cytoplasm, perinuclear region. Mitochondrion.
组织特异性：

Ubiquitous. Isoform 2 is highly expressed in testis and lung, but detected at low levels in thymus, prostate, colon and liver.
数据库链接：

HGNC: 5229

OMIM: 602837

KEGG: hsa:3301

STRING: 9606.ENSP00000369127

UniGene: Hs.445203

DNAJA1 Recombinant Monoclonal Antibody

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Application	Recommended Dilution
WB	1:500-1:2000
IF	1:50-1:200
FC	1:50-1:200