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中文名称:Claudin-18.2重组抗体
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货号:CSB-RA005498A1HU
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规格:¥1320
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图片:
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Untransfected KATO3 cells surface (green line), transfected Human CLDN18.2 KATO3 stable cells surface (red line) and transfected Human CLDN18.1 NIH-3T3 stable cells surface (blue line) were stained with anti-CLDN18.2 antibody (2µg/1*106 cells), washed and then followed by FITC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.
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The Binding Activity of Human CLDN18.2 with Anti-CLDN18.2 recombinant antibody.
Activity: Measured by its binding ability in a functional ELISA. Immobilized Human CLDN18.2 (CSB-MP005498HU(A5)) at 5 μg/mL can bind Anti-CLDN18.2 recombinant antibody, the EC50 is 6.554-27.87 ng/mL.
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其他:
产品详情
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产品描述:CSB-RA005498A1HU Claudin-18.2重组单克隆抗体是针对紧密连接蛋白家族成员Claudin-18.2研发的高特异性科研试剂。该靶点通过调控上皮细胞间紧密连接参与屏障功能维持,其亚型Claudin-18.2在胃癌、胰腺癌等消化道肿瘤中呈现特异性高表达,成为肿瘤生物学研究的重要生物标志物。本抗体经ELISA和流式细胞术(FC)双重验证,在FC应用中推荐使用1:50-1:200的稀释范围,可精准识别天然构象的膜表面抗原,适用于活细胞或固定化样本的定量检测。该产品凭借优异的批间一致性和低交叉反应特性,可支持肿瘤细胞表面标记物分析、靶向药物体外筛选模型构建以及肿瘤微环境中细胞亚群分选等研究场景,为探索Claudin-18.2在肿瘤发生、转移及耐药机制中的功能提供可靠工具,助力抗肿瘤药物开发及转化医学研究。本产品严格限定于体外实验及基础科研用途。
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Uniprot No.:
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基因名:
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别名:Claudin-18, CLDN18
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反应种属:Human
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免疫原:Recombinant Human Claudin-18.2 protein
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免疫原种属:Homo sapiens (Human)
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标记方式:Non-conjugated
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克隆类型:Monoclonal
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抗体亚型:hIgG1
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纯化方式:Affinity-chromatography
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克隆号:9C2
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浓度:It differs from different batches. Please contact us to confirm it.
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保存缓冲液:Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 -
产品提供形式:Liquid
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应用范围:ELISA, FC
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推荐稀释比:
Application Recommended Dilution FC 1:50-1:200 -
Protocols:
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储存条件:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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货期:Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途:For Research Use Only. Not for use in diagnostic or therapeutic procedures.
产品评价
实验应用:IHC, FC, ELISA
样品信息: 人胃黏膜细胞
稀释比:1:100
产品评价: 我使用CSB-RA005498A1HU的抗体,进行ELISA和WB检测人的细胞,WB检测结果条带较单一,检测条带大小与预期的一致,ELISA检测EC50 为2.623-14.06 ng/mL。抗体特异性好,灵敏度高。
By 彭老师
实验应用:FC
样品信息:人来源细胞
稀释比:1.100
产品评价: 正常流式过程。
By 付老师
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