CA1 Recombinant Monoclonal Antibody
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中文名称:CA1重组抗体
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货号:CSB-RA217389A0HU
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规格:¥1320
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图片:
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Western Blot
Positive WB detected in: K562 whole cell lysate,U937 whole cell lysate
All lanes: Ikaros antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDa -
IHC image of CSB-RA217389A0HU diluted at 1:50 and staining in paraffin-embedded human spleen tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.36% DAB.
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Overlay Peak curve showing MCF-7 cells stained with CSB-RA217389A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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其他:
产品详情
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产品描述:CA1 Recombinant Monoclonal Antibody(CSB-RA217389A0HU)是一款针对碳酸酐酶1(CA1)的高特异性科研工具,适用于多种实验场景。CA1作为碳酸酐酶家族成员,广泛参与酸碱平衡调节及二氧化碳代谢过程,其表达异常与代谢性疾病、肿瘤微环境调控等研究领域密切相关。本抗体经ELISA、Western Blot(WB)、免疫组织化学(IHC)和流式细胞术(FC)严格验证:在WB中可检测到清晰靶蛋白条带,推荐稀释比例1:500-1:2000;IHC实验显示其在石蜡包埋组织中具有明确的亚细胞定位能力,推荐使用浓度1:50-1:200;FC验证证实其适用于细胞表面或胞内CA1蛋白的检测,建议稀释范围1:50-1:200。该抗体可应用于细胞代谢机制研究、疾病模型构建及组织病理学分析等科研方向,其重组单克隆特性确保了批次间的高度一致性,并经过抗原亲和纯化以降低非特异性背景。实验时建议根据样本类型优化具体条件,配套设立阳性和阴性对照以确保结果可靠性。
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Uniprot No.:
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基因名:
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别名:Carbonic anhydrase 1 (EC 4.2.1.1) (Carbonate dehydratase I) (Carbonic anhydrase B) (CAB) (Carbonic anhydrase I) (CA-I), CA1
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反应种属:Human
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免疫原:A synthesized peptide derived from Human CA1
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免疫原种属:Homo sapiens (Human)
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标记方式:Non-conjugated
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克隆类型:Monoclonal
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抗体亚型:Rabbit IgG
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纯化方式:Affinity-chromatography
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克隆号:26E3
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浓度:It differs from different batches. Please contact us to confirm it.
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保存缓冲液:Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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产品提供形式:Liquid
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应用范围:ELISA, WB, IHC, FC
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推荐稀释比:
Application Recommended Dilution WB 1:500-1:2000 IHC 1:50-1:200 FC 1:50-1:200 -
Protocols:
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储存条件:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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货期:Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途:For Research Use Only. Not for use in diagnostic or therapeutic procedures.
相关产品
靶点详情
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功能:Reversible hydration of carbon dioxide. Can hydrates cyanamide to urea.
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基因功能参考文献:
- Anti-CA I and II antibody levels were investigated using ELISA in serum samples from 30 patients with Acute Myeloid Leukemia (AML) and 30 healthy peers. Anti-CA I and II antibody titers in the AML group were significantly higher compared with the control group (p=0.0001 and 0.018, respectively). PMID: 28270370
- These six-membered carbocycles showed nice inhibitory action against AChE and human carbonic anhydrase (hCA) II and I isoforms. The hCA I, II, and AChE were efficiently inhibited by these molecules, with Ki values in the range of 6.70-35.85 nM for hCA I, 18.77-60.84 nM for hCA II, and 0.74-4.60 for AChE, respectively PMID: 28613396
- These results showed that flavonoids especially malvin and oenin effectively inhibited hCA I and II isoenzymes. PMID: 28445001
- Discovery of potent carbonic anhydrase, acetylcholinesterase, and butyrylcholinesterase enzymes inhibitors: The new amides and thiazolidine-4-ones synthesized on an acetophenone base. PMID: 28544359
- Here are presented solved the first crystal structures of carbonic anhydrase 1and carbonic anhydrase 2 in complex with polmacoxib, at 2.0 A and 1.8 A, respectively. PMID: 27475498
- Neuronal carbonic anhydrase I (CA1) appears to be associated with the endoplasmic reticulum subcellular structure. PMID: 27809276
- CA1 is highly expressed in the sera of stage I non-small cell lung cancer patients PMID: 26232327
- We demonstrated that resveratrol, caffeic acid, and tannic acid in stored blood could decrease the sensitivity to oxidation of erythrocytes in vitro but did not exhibit such effects on Carbonic Anhydrase activity. PMID: 27413740
- CA1 is a potential oncogene and that it contributes to abnormal cell calcification, apoptosis and migration in breast cancer. PMID: 26459317
- We report on the competitive zinc metalation of apo-carbonic anhydrase [CA; metal-free CA (apo-CA)] in the presence of apo-metallothionein 1A domain fragments to identify domain specific determinants of zinc binding and zinc donation PMID: 26475450
- The gene encoding CA1 is susceptible to ankylosing spondylitis and plays a role in the process of bone formation. PMID: 22838845
- Structure-activity relationships study showed that indolylchalcone derivatives have higher inhibitory activities than pyrido[2,3-d]pyrimidine derivatives on hCA I and hCA II. PMID: 25165709
- Myocardial carbonic anhydrase 1/2 activation is significantly elevated in diabetic ischemic cardiomyopathy. PMID: 24670789
- may be involved in the pathogenesis of Abdominal aortic aneurysm PMID: 23557951
- The new prognostic biomarkers GRP78, Fructose-bisphosphate Aldolase A (ALDOA), Carbonic Anhydrase I (CA1) and Peptidyl-prolyl cis-trans isomerase A or Cyclophilin A (PPIA)) provided good survival prediction for TNM stage I-IV patients. PMID: 22996014
- A significant correlation was found between positive carbonic anhydrase I staining and oral squamous cell carcinoma for more advanced clinical stage and larger tumor size, but not for positive lymph node metastasis, distal metastasis, and recurrence. PMID: 22416960
- carbonic anhydrase I, phosphoglycerate kinase 1 and apolipoprotein A-I appeared to be the most significant variations of proteins in patients with osteopenia and osteoarthritis PMID: 22619369
- A protein encoded by this locus was found to be differentially expressed in postmortem brains from patients with atypical frontotemporal lobar degeneration. PMID: 22360420
- Overexpression of CA1 in the synovial tissues of ankylosing spondylitis (AS) patients may promote improper calcification and bone resorption in AS. PMID: 21143847
- single mutation of an amino acid not considered essential to catalysis (Phe91Asn) in carbonic anhydrase I, but is near substrate binding pocket, led to increase of catalytic activity; mutant CA I also showed higher affinity for sulfonamide inhibitors PMID: 20624682
- The X-ray crystallographic structure of the CA I Michigan 1 variant isozyme is reported, both in the presence and absence of a second bound zinc ion coordinated to His 64, His 200, and Arg 67. PMID: 12009884
- concentrations in nondialyzed chronic kidney disease patients, and the relationship with acidosis, zinc, anemia, and iron supplementation PMID: 14675565
- cytosolic CA I, II, and XIII are downregulated in neoplastic colorectal mucosa compared to normal colorectal mucosa PMID: 15836783
- Isothiocyanato sulfonamide thioureas inhibit this enzyme. PMID: 15837325
- X-ray crystallographic structure for the adduct of an activator with hCA I. Binding site interactions of activator L-Histidine with active site amino acids PMID: 16870440
- study presents the X-ray structure of the foscarnet adduct with CA1 together with the factors governing recognition of such small molecules by a metalloenzyme active site PMID: 17314045
- Decreased levels of carbonic anhydrase 1 isozyme is associated with type II diabetes. PMID: 17464559
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亚细胞定位:Cytoplasm.
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蛋白家族:Alpha-carbonic anhydrase family
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