AKT1/2/3 Recombinant Monoclonal Antibody

中文名称：

AKT1/2/3重组抗体
货号：

CSB-RA272392A0HU
规格：

￥1320
图片：
Western Blot
Positive WB detected in: SH-SY5Y whole cell lysate
All lanes: CD44 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 56 kDa
Observed band size: 56 kDa

IHC image of CSB-RA272392A0HU diluted at 1:50 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.54% DAB.

Overlay Peak curve showing Jurkat cells stained with CSB-RA272392A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1µg/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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产品描述：

AKT1/2/3 Recombinant Monoclonal Antibody（Code: CSB-RA272392A0HU）是一款经多平台验证的高特异性抗体，靶向AKT激酶家族成员（AKT1、AKT2、AKT3）。AKT蛋白在PI3K-AKT-mTOR信号通路中发挥核心作用，参与调控细胞增殖、存活和代谢等关键生物学过程，其异常激活与肿瘤发生、耐药性及代谢性疾病密切相关。本抗体采用重组单克隆技术开发，可精准识别内源性AKT蛋白，经严格验证适用于ELISA、Western Blot（推荐稀释1:500-1:2000）、免疫组化（推荐稀释1:50-1:200）和流式细胞术（推荐稀释1:50-1:200）。实验数据显示其在不同样本类型（如细胞裂解物、石蜡包埋组织切片及悬浮细胞）中均能呈现清晰的检测信号，Western Blot检测可区分目标蛋白的预期分子量，免疫组化染色定位准确且背景低，流式分析灵敏度满足细胞表面及胞内标记需求。该产品适用于癌症机制研究、信号通路互作分析、药物靶点筛选及代谢调控相关基础科研，其高批次一致性为实验重复性提供可靠保障，助力研究者深入探索AKT蛋白在疾病模型中的功能与调控网络。
Uniprot No.：

P31749 P31751 Q9Y243
基因名：

AKT1/2/3
别名：

RAC-alpha serine/threonine-protein kinase (EC 2.7.11.1) (Protein kinase B) (PKB) (Protein kinase B alpha) (PKB alpha) (Proto-oncogene c-Akt) (RAC-PK-alpha), AKT1, PKB RAC, RAC-beta serine/threonine-protein kinase (EC 2.7.11.1) (Protein kinase Akt-2) (Protein kinase B beta) (PKB beta) (RAC-PK-beta), AKT2, RAC-gamma serine/threonine-protein kinase (EC 2.7.11.1) (Protein kinase Akt-3) (Protein kinase B gamma) (PKB gamma) (RAC-PK-gamma) (STK-2), AKT3, PKBG
反应种属：

Human
免疫原：

A synthesized peptide derived from Human AKT1/2/3 Antibody
免疫原种属：

Homo sapiens (Human)
标记方式：

Non-conjugated
克隆类型：

Monoclonal
抗体亚型：

Rabbit IgG
纯化方式：

Affinity-chromatography
克隆号：

16E10
浓度：

It differs from different batches. Please contact us to confirm it.
保存缓冲液：

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
产品提供形式：

Liquid
应用范围：

ELISA, WB, IHC, FC
推荐稀释比：

Application Recommended Dilution

WB 1:500-1:2000

IHC 1:50-1:200

FC 1:50-1:200
Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Flow cytometry (FC) Protocol
储存条件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
货期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

AKT1/2/3 Recombinant Monoclonal Antibody

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Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
FC	1:50-1:200