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  4. ACAA2 Recombinant Monoclonal Antibody

ACAA2 Recombinant Monoclonal Antibody

说明书
  • 中文名称：
    ACAA2重组抗体
  • 货号：
    CSB-RA978277A0HU
  • 规格：
    ￥1320
  • 图片：
    • Western Blot
      Positive WB detected in: Hela whole cell lysate(20µg), A549 whole cell lysate(20µg), HepG2 whole cell lysate(20µg), 293T whole cell lysate(20µg), MCF7 whole cell lysate(20µg), JK whole cell lysate(20µg), Mouse Liver tissue lysate(20µg), Mouse Kidney tissue lysate(20µg)
      All lanes: ACAA2 antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 42 kDa
      Observed band size: 42 kDa
      Exposure time: 10s
    • IHC image of CSB-RA978277A0HU diluted at 1:150 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody
    • IHC image of CSB-RA978277A0HU diluted at 1:150 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody
    • IHC image of CSB-RA978277A0HU diluted at 1:150 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody
    • Immunofluorescence staining of Hela cell with CSB-RA978277A0HU at 1:12, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品描述：
    CSB-RA978277A0HU ACAA2重组单克隆抗体是针对乙酰辅酶A酰基转移酶2（Acetyl-CoA acyltransferase 2）靶点开发的高特异性科研工具，该酶是过氧化物酶体脂肪酸β氧化途径中的关键硫解酶，参与中长链脂肪酸代谢调控，与脂质稳态及细胞能量代谢密切相关。本产品经多种实验验证，可在Western Blot中呈现单一清晰条带，并在免疫组化与免疫荧光实验中显示明确的亚细胞定位，推荐使用稀释度为WB 1:500-1:2000、IHC/IF 1:50-1:200，所有批次的灵敏度与特异性均通过严格的质控验证，确保实验结果的稳定性和可重复性。适用于探究ACAA2在代谢性疾病模型、脂质蓄积相关机制及过氧化物酶体功能研究中的表达调控，为体外细胞实验或组织样本的蛋白水平分析提供可靠支持，助力于能量代谢通路与相关病理生理机制的分子生物学研究。
  • Uniprot No.：
    P42765
  • 基因名：
    ACAA2
  • 别名：
    3-ketoacyl-CoA thiolase, mitochondrial (EC 2.3.1.16) (Acetyl-CoA acyltransferase) (Beta-ketothiolase) (Mitochondrial 3-oxoacyl-CoA thiolase) (T1), ACAA2
  • 反应种属：
    Human, Mouse
  • 免疫原：
    A synthesized peptide derived from human ACAA2
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    12F4
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:2000
    IHC 1:50-1:200
    IF 1:10-1:100
  • Protocols：
    ELISA Protocol
    Western Blotting (WB) Protocol
    Immunohistochemistry (IHC) Protocol
    Immunofluorescence (IF) Protocol
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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ACAA2 antibodies

ACAA2 Proteins

靶点详情

  • 功能：
    In the production of energy from fats, this is one of the enzymes that catalyzes the last step of the mitochondrial beta-oxidation pathway, an aerobic process breaking down fatty acids into acetyl-CoA. Using free coenzyme A/CoA, catalyzes the thiolytic cleavage of medium- to long-chain unbranched 3-oxoacyl-CoAs into acetyl-CoA and a fatty acyl-CoA shortened by two carbon atoms. Also catalyzes the condensation of two acetyl-CoA molecules into acetoacetyl-CoA and could be involved in the production of ketone bodies. Also displays hydrolase activity on various fatty acyl-CoAs. Thereby, could be responsible for the production of acetate in a side reaction to beta-oxidation. Abolishes BNIP3-mediated apoptosis and mitochondrial damage.
  • 基因功能参考文献：
    1. ACAA2 is a functional BNIP3 binding partner and provide a possible linkage between fatty acid metabolism and apoptosis of cells. PMID: 18371312
  • 亚细胞定位：
    Mitochondrion.
  • 蛋白家族：
    Thiolase family
  • 数据库链接：

    HGNC: 83

    OMIM: 604770

    KEGG: hsa:10449

    STRING: 9606.ENSP00000285093

    UniGene: Hs.200136

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