ligA Antibody, Biotin conjugated

^{Rare Species}

产品详情

产品名称：

Rabbit anti-Escherichia coli ligA Polyclonal antibody
Uniprot No.：

P15042
基因名：

ligA
别名：

ligA antibody; dnaL antibody; lig antibody; lop antibody; pdeC antibody; b2411 antibody; JW2403 antibody; DNA ligase antibody; EC 6.5.1.2 antibody; Polydeoxyribonucleotide synthase [NAD(+)] antibody
宿主：

Rabbit
反应种属：

Escherichia coli
免疫原：

Recombinant Escherichia coli DNA ligase protein (1-671AA)
免疫原种属：

Escherichia coli
标记方式：

Biotin
克隆类型：

Polyclonal
抗体亚型：

IgG
纯化方式：

>95%, Protein G purified
浓度：

It differs from different batches. Please contact us to confirm it.
保存缓冲液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
产品提供形式：

Liquid
应用范围：

ELISA
Protocols：

ELISA Protocol
储存条件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
货期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

功能：

DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA.
基因功能参考文献：
1. Kinetic mechanism and fidelity of nick sealing by Escherichia coli LigA has been described. PMID: 26857547
2. Here the authors show that replication without ligase kills after a short resistance period. PMID: 22582878
3. temperature sensitive mutant characterized PMID: 15583169
4. analysis of the nucleotidyltransferase domain of Escherichia coli NAD+-dependent DNA ligase (LigA) PMID: 15671015
5. The deletion of the BRCT domain from LigA reduced in vitro ligation activity by 3-fold and also reduced DNA binding. PMID: 15848142
6. 2.3 A crystal structure of LigA bound to an adenylylated nick, which captures LigA in a state poised for strand closure and reveals the basis for nick recognition PMID: 17466627
7. structure-function analysis of the OB, HhH & BRCT domains of LigA; study found that the BRCT domain is required in its entirety for effective nick sealing and AMP-dependent supercoil relaxation PMID: 18515356
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蛋白家族：

NAD-dependent DNA ligase family, LigA subfamily
数据库链接：

KEGG: ecj:JW2403

STRING: 316385.ECDH10B_2576