IRF2BP1 Antibody

中文名称：

IRF2BP1兔多克隆抗体
货号：

CSB-PA815541LA01HU
规格：

￥440
图片：
IHC image of CSB-PA815541LA01HU diluted at 1:100 and staining in paraffin-embedded human salivary gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of Hela cells with CSB-PA815541LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
其他：

产品详情

产品名称：

Rabbit anti-Homo sapiens (Human) IRF2BP1 Polyclonal antibody
Uniprot No.：

Q8IU81
基因名：

IRF2BP1
别名：

DKFZp434M154 antibody; I2BP1_HUMAN antibody; Interferon regulatory factor 2 binding protein 1 antibody; Interferon regulatory factor 2-binding protein 1 antibody; IRF 2BP1 antibody; IRF-2-binding protein 1 antibody; IRF-2BP1 antibody; IRF2 binding protein 1 antibody; Irf2bp1 antibody
宿主：

Rabbit
反应种属：

Human
免疫原：

Recombinant Human Interferon regulatory factor 2-binding protein 1 protein (340-499AA)
免疫原种属：

Homo sapiens (Human)

标记方式：

Non-conjugated

本页面中的产品，IRF2BP1 Antibody (CSB-PA815541LA01HU)，的标记方式是Non-conjugated。对于IRF2BP1 Antibody，我们还提供其他标记。见下表：

标记方式	货号	产品名称	应用
HRP	CSB-PA815541LB01HU	IRF2BP1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA815541LC01HU	IRF2BP1 Antibody, FITC conjugated
Biotin	CSB-PA815541LD01HU	IRF2BP1 Antibody, Biotin conjugated	ELISA

克隆类型：

Polyclonal
抗体亚型：

IgG
纯化方式：

>95%, Protein G purified
浓度：

It differs from different batches. Please contact us to confirm it.
保存缓冲液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
产品提供形式：

Liquid
应用范围：

ELISA, IHC, IF
推荐稀释比：

Application Recommended Dilution

IHC 1:20-1:200

IF 1:50-1:200
Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
储存条件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
货期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

功能：

Acts as a transcriptional corepressor in a IRF2-dependent manner; this repression is not mediated by histone deacetylase activities. May act as an E3 ligase towards JDP2, enhancing its polyubiquitination. Represses ATF2-dependent transcriptional activation.
基因功能参考文献：
1. Identification of IRF-2 binding protein 1 and IRF-2 binding protein 2 as co-repressor molecules for interferon regulatory factor-2. PMID: 12799427
2. PCTA (IRF2BP1) defines a new component of the TGF-beta signalling pathway that functions to facilitate Smad2 phosphorylation through controlling the accumulation of cPML into the cytoplasm, and consequently, the assembly of Smad2-receptor complex. PMID: 18511908
3. IRF2-BP1 is a JDP2-binding protein enhancing the polyubiquitination of JDP2 and represses ATF2-mediated transcriptional activation from a CRE-containing promoter. PMID: 18671972
亚细胞定位：

Nucleus.
蛋白家族：

IRF2BP family
数据库链接：

HGNC: 21728

OMIM: 615331

KEGG: hsa:26145

STRING: 9606.ENSP00000307265

UniGene: Hs.515477

Application	Recommended Dilution
IHC	1:20-1:200
IF	1:50-1:200