HLA-C Antibody

中文名称：

HLA-C兔多克隆抗体
货号：

CSB-PA15099A0Rb
规格：

￥440
图片：
IHC image of CSB-PA15099A0Rb diluted at 1:400 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody

Immunofluorescence staining of Hela cell with CSB-PA15099A0Rb at 1:80, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Western Blot
Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, A431 whole cell lysate
All lanes: HLA-C antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 41 kDa
Observed band size: 41 kDa
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产品描述：

HLA-C polyclonal antibody CSB-PA15099A0Rb was produced in the rabbit immunized by using the Recombinant Human HLA-C protein (25-308AA) as the immunogen. The target protein HLA-C belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain. Class I molecules play a critical role in the immune system by presenting peptides derived from endoplasmic reticulum lumen. Diseases associated with HLA-C include Psoriasis 1 and Human Immunodeficiency Virus Type 1.
This Rabbit anti-Homo sapiens (Human) HLA-C Polyclonal antibody was tested in the ELISA, WB, IHC and IF applications. The non-conjugated IgG got purified by protein G and reached up to 95% in purity. It only reacts with the HLA-C proteins of human-origin and may be used to detect the endogenous levels of HLA-C protein.
Uniprot No.：

O78179
宿主：

Rabbit
反应种属：

Human
免疫原：

Recombinant Human HLA-C protein (25-308AA)
免疫原种属：

Homo sapiens (Human)

标记方式：

Non-conjugated

本页面中的产品，HLA-C Antibody (CSB-PA15099A0Rb)，的标记方式是Non-conjugated。对于HLA-C Antibody，我们还提供其他标记。见下表：

标记方式	货号	产品名称	应用
HRP	CSB-PA15099B0Rb	HLA-C Antibody, HRP conjugated	ELISA
FITC	CSB-PA15099C0Rb	HLA-C Antibody, FITC conjugated
Biotin	CSB-PA15099D0Rb	HLA-C Antibody, Biotin conjugated	ELISA

克隆类型：

Polyclonal
抗体亚型：

IgG
纯化方式：

>95%, Protein G purified
浓度：

It differs from different batches. Please contact us to confirm it.
保存缓冲液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
产品提供形式：

Liquid
应用范围：

ELISA, WB, IHC, IF
推荐稀释比：

Application Recommended Dilution

WB 1:1000-1:5000

IHC 1:200-1:500

IF 1:50-1:200
Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
储存条件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
货期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

HLA-C Antibody

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Application	Recommended Dilution
WB	1:1000-1:5000
IHC	1:200-1:500
IF	1:50-1:200