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  4. ATP9A Antibody

ATP9A Antibody

说明书
  • 中文名称：
    ATP9A兔多克隆抗体
  • 货号：
    CSB-PA002422LA01HU
  • 规格：
    ￥3,900
  • 图片：
    • IHC image of CSB-PA002422LA01HU diluted at 1:50 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody
    • Immunofluorescence staining of A549 cell with CSB-PA002422LA01HU at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Immunofluorescence staining of A549 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Western Blot
      Positive WB detected in: A549 whole cell lysate, Hela whole cell lysate, SY5Y whole cell lysate
      All lanes: ATP9A antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 118 kDa
      Observed band size: 118 kDa
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) ATP9A Polyclonal antibody
  • Uniprot No.：
    O75110
  • 基因名：
    ATP9A
  • 别名：
    ATP 9A antibody; Atp9a antibody; ATP9A_HUMAN antibody; ATPase class II type 9A antibody; ATPase IIA antibody; ATPase type IV phospholipid transporting (P type) antibody; ATPase type IV phospholipid transporting (P type)(putative) antibody; ATPIIA antibody; KIAA0611 antibody; Phospholipid transporting ATPase IIA antibody; Probable phospholipid transporting ATPase IIA antibody; Probable phospholipid-transporting ATPase IIA antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Probable phospholipid-transporting ATPase IIA protein
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，ATP9A Antibody (CSB-PA002422LA01HU)，的标记方式是Non-conjugated。对于ATP9A Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA002422LB01HU ATP9A Antibody, HRP conjugated ELISA
    FITC CSB-PA002422LC01HU ATP9A Antibody, FITC conjugated
    Biotin CSB-PA002422LD01HU ATP9A Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    Antigen affinity purification
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.02% sodium azide
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:1000-1:3000
    IHC 1:50-1:200
    IF 1:20-1:100
  • Protocols：
    ELISA Protocol
    Western Blotting (WB) Protocol
    Immunohistochemistry (IHC) Protocol
    Immunofluorescence (IF) Protocol
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

产品评价

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ATP9A antibodies

ATP9A Proteins

靶点详情

  • 功能：
    Plays a role in regulating membrane trafficking of cargo proteins, namely endosome to plasma membrane recycling and endosome to trans-Golgi network retrograde transport. In complex with MON2 and DOP1B, regulates SNX3 retromer-mediated endosomal sorting of WLS, a transporter of Wnt morphogens in developing tissues. Participates in the formation of endosomal carriers that direct WLS trafficking back to Golgi, away from lysosomal degradation. Appears to be implicated in intercellular communication by negatively regulating the release of exosomes. The flippase activity towards membrane lipids and its role in membrane asymmetry remains to be proved.
  • 基因功能参考文献：
    1. the predominant P4 ATPases in pure pancreatic beta cells and human and rat pancreatic islets were ATP8B1, ATP8B2, and ATP9A. ATP8B1 and CDC50A were highly concentrated in ISG PMID: 26240149
    2. Clinical trial and genome-wide association study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator) PMID: 18519826
  • 亚细胞定位：
    Early endosome membrane; Multi-pass membrane protein. Recycling endosome membrane; Multi-pass membrane protein. Golgi apparatus, trans-Golgi network membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Cation transport ATPase (P-type) (TC 3.A.3) family, Type IV subfamily
  • 数据库链接：

    HGNC: 13540

    OMIM: 609126

    KEGG: hsa:10079

    STRING: 9606.ENSP00000342481

    UniGene: Hs.649234

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