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ADAM8 Antibody

说明书
  • 中文名称：
    ADAM8兔多克隆抗体
  • 货号：
    CSB-PA10259A0Rb
  • 规格：
    ￥440
  • 图片：
    • Western Blot
      Positive WB detected in: THP-1 whole cell lysate(20µg), K562 whole cell lysate(20µg), JK whole cell lysate(20µg)
      All lanes: ADAM8 antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 89, 79,81 kDa
      Observed band size: 100 kDa
      Exposure time: 300s
    • ImmunofluorescencestainingofHelacellwithCSB-PA10259A0Rbat1:30,counter-stainedwithDAPI.Thecellswerefixedin4%formaldehydeandblockedin10%normalGoatSerum.Thecellswerethenincubatedwiththeantibodyovernightat4C.ThesecondaryantibodywasAlexaFluor488-congugatedAffiniPureGoatAnti-RabbitIgG(H+L).
    • Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Immunofluorescence staining of MCF-7 cell with CSB-PA10259A0Rb at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • ImmunofluorescencestainingofMCF-7cellwith5%goatserum,counter-stainedwithDAPI.Thecellswerefixedin4%formaldehydeandblockedin10%normalGoatSerum.Thecellswerethenincubatedwiththeantibodyovernightat4C.ThesecondaryantibodywasAlexaFluor488-congugatedAffiniPureGoatAnti-RabbitIgG(H+L).
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) ADAM8 Polyclonal antibody
  • Uniprot No.：
    P78325
  • 基因名：
    ADAM8
  • 别名：
    A Disintegrin And Metalloproteinase domain 8 antibody; ADAM 8 antibody; ADAM 8 precursor antibody; ADAM metallopeptidase domain 8 antibody; Adam8 antibody; ADAM8 protein antibody; ADAM8_HUMAN antibody; CD 156 antibody; CD156 antibody; CD156a antibody; CD156a antigen antibody; Cell surface antigen MS2 antibody; Disintegrin and metalloproteinase domain-containing protein 8 antibody; Human leukocyte differentiation antigen antibody; Macrophage cysteine rich glycoprotein antibody; MGC134985 antibody; MS 2 antibody; MS2 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Synthesized peptide derived from part region of human protein
  • 免疫原种属：
    Homo sapiens (Human)
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    Affinity-purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IF 1:50-1:200
  • Protocols：
    ELISA Protocol
    Western Blotting (WB) Protocol
    Immunofluorescence (IF) Protocol
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

产品评价

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ADAM8 antibodies

ADAM8 Proteins

靶点详情

  • 功能：
    Possible involvement in extravasation of leukocytes.
  • 基因功能参考文献：
    1. Authors conclude that ADAM8 promotes early metastatic processes such as transendothelial migration by upregulation of MMP-9 and shedding of PSGL-1 from breast cancer cells. PMID: 28986926
    2. ADAM8 on leukocytes holds a proinflammatory function in acute lung inflammation by promoting alveolar leukocyte recruitment. PMID: 28596294
    3. miR-720 is elevated in serum of patients with ADAM8-high TNBC and, in a group with other miRNAs downstream of ADAM8, holds promise as a biomarker for early detection of or treatment response of ADAM8-positive triple-negative breast cancer PMID: 27039296
    4. ADAM8 causes temozolomide resistance in glioblastoma cells by enhancing pAkt/PI3K, pERK1/2, and cleavage of CD44 and HGF R/c-met PMID: 25825051
    5. ADAM8 promotes GC cell proliferation and invasion, and its expression is positively correlated with poor survival, indicating that it might be a promising target in GC therapy PMID: 26024798
    6. ADAM8 expression is associated with increased migration and invasiveness of pancreatic ductal adenocarcinoma cells. PMID: 25629724
    7. ADAM8 and endostatin play a role in osteosarcoma progression. PMID: 25481287
    8. Data indicate that fibronectin fragments (FN-fs) are present in adult ntervertebral disc (IVD) from adult subjects, and ADAM-8, known to cleave FN, is present at the pericellular matrix of disc cells. PMID: 25010013
    9. Results show that ADAM8 is overexpressed in colorectal cancer and promotes cell growth. PMID: 25098630
    10. N-glycosylation is essential for processing, localization, stability, and activity of ADAM8. PMID: 25336660
    11. ADAM8 expression is increased in both severe asthma and COPD and associated with sputum total cell count and neutrophils. ADAM8 may facilitate neutrophil migration to the airways in severe asthma and COPD. PMID: 24147597
    12. ADAM8 is abundantly expressed in breast tumors and derived metastases compared to normal tissue. PMID: 24375628
    13. High ADAM8 expression is associated with pancreatic adenocarcinoma. PMID: 24526468
    14. Data show that Cu2+-generated reactive oxygen species (ROS), human ADAM8, ADAM10, and ADAM17 are all capable of cleaving mouse PrP (MoPrP). PMID: 24247244
    15. ADAM8 was highly expressed in 54.3% of hepatocellular carcinoma patients. ADAM8 expression was closely associated with tumor size, histological differentiation, recurrence, metastasis, and stage. High levels of ADAM8 resulted in poor prognosis. PMID: 22878099
    16. We used immunohistochemistry to compare ADAM8 protein expression in HCC and normal liver tissues and further analyze the ADAM8 protein expression in 105 HCC cases. Studied knocked down expression of ADAM8 in HepG2 cells. PMID: 22965687
    17. ADAM8 was robustly expressed by airway granulocytes in lung sections from human asthma patients PMID: 23670189
    18. These findings suggest for the first time that ADAM8 is frequently overexpressed in human gliomas and is closely associated with poor clinical outcome. PMID: 21983884
    19. ADAM8 mRNA & protein were highly expressed in medulloblastoma tissues when compared with normal cerebellum. This correlated with advanced stage, aggressive type, undifferentiated tumor & decreased survival. PMID: 22959284
    20. High expression of ADAM8 is associated with hepatocellular carcinoma. PMID: 22941466
    21. Upregulation of a disintegrin and metalloprotease 8 influences tumor metastasis with osteosarcoma. PMID: 22215309
    22. variation in the ADAM8 gene may affect serum sADAM8 concentrations and the risk of myocardial infarction PMID: 21640993
    23. ADAM8 is both associated with PSGL-1 through the ezrin-radixin-moesin actin-binding proteins and able to cause the proteolytic cleavage of this adhesion receptor. PMID: 22229154
    24. gene expressions for ADAM8 and ADAM15 were notably lower in ascending aorta as compared with aortic dissection PMID: 21728902
    25. increased expression in allergic rhinitis PMID: 21679521
    26. It regulates onset of blood circulation. (review) PMID: 21077325
    27. ADAM8 is a promising candidate to be involved in atherosclerosis, and its 2662 T/G allelic variant significantly associates with advanced atherosclerotic lesion areas and myocardial infarction. PMID: 19575316
    28. ADAM8 and EGFR are overexpressed in non-small cell lung cancer. PMID: 18710625
    29. expression of truncated forms of ADAM8 by the lung cancer cells may result in the specific upregulation of their invasive and osteoclastogenic activities in the bone microenvironment PMID: 20453887
    30. Overexpression of ADAM8 is associated with lung cancer progression PMID: 15623614
    31. ADAM8 may contribute to the airway remodeling process that occurs with asthma progression. PMID: 17339047
    32. All these data support a potential relevant role for ADAM-8 in the function of neutrophils during inflammatory response. PMID: 17548643
    33. ADAM8 is overexpressed in pancreatic ductal adenocarcinoma, influences cancer cell invasiveness and correlates with reduced survival. PMID: 17979891
    34. ADAM8 may have a role as a hypoxia-dependent protein in the pathogenesis and evolution of pancreatic cancer PMID: 18566576
    35. ADAM8 might be a therapeutic target for allergic respiratory diseases. PMID: 18691140
    36. Autoactivation of ADAM8: a novel pre-processing step is required for catalytic activity. PMID: 18811590
    37. ADAM8 is up-regulated upon formation of multinuclear giant cells after HPIV2 induction. PMID: 19284887
    38. The structure, function & expression of ADAM8 and its role in asthma are reviewed. Review. PMID: 19397475
    39. Increasing trend for ADAM8 expression is associated with early to advanced stages of myelodysplastic syndrome. PMID: 19469654
    40. ADAM-8 as a fibronectinase in human osteoarthritis chondrocytes. PMID: 19714641
    41. the effects of pH on substrate cleavage by ADAM8 beyond autocatalysis PMID: 19766586

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  • 亚细胞定位：
    Membrane; Single-pass type I membrane protein.
  • 组织特异性：
    Expressed on neutrophils and monocytes.
  • 数据库链接：

    HGNC: 215

    OMIM: 602267

    KEGG: hsa:101

    STRING: 9606.ENSP00000453302

    UniGene: Hs.501574

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