Recombinant Mouse Alkaline phosphatase, germ cell type (Alpg) (Active)

Greater than 95% as determined by SDS-PAGE.
Greater than 90% as determined by SEC-HPLC.

Less than 1.0 EU/ug as determined by LAL method.

Unit Definition:One unit is defined as the amount of enzyme required to cleave 1 nmol p-nitro-phenylphosphate(pNPP), in 1 minute at 37°C, pH10.0.The specific activity is >6000 U/mg.

P24823

Alkaline phosphatase, germ cell type; EC:3.1.3.1; Alkaline phosphatase 5; Alkaline phosphatase; placental-like; Embryonic alkaline phosphatase; Embryonic-type alkaline phosphatase

Mus musculus (Mouse)

Full Length of Mature Protein

Mammalian cell

53.9kDa

19-502aa

C-terminal 10xHis-tagged

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

3-7 business days

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

Please contact us to get it.