Whitlow linker Monoclonal Antibody

中文名称：

Whitlow linker CAR检测工具
货号：

CSB-MA234468A0m
规格：

￥3000
图片：
The Binding Activity of Whitlow linker with Anti-Whitlow linker antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized scFv-based Anti-CD19 CAR recombinant antibody at 2 μg/mL can bind Anti-Whitlow linker antibody. The EC₅₀ is 5.147 to 5.761 ng/mL.

Western Blot
Positive WB detected in: 293F whole cell lysate, scFv-based Anti-CD19 CAR containing a Whitlow linker (293F/CD19 CAR) whole cell lysate
All lanes: Whitlow linker antibody at 1:1000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 50-200 kDa
Observed band size: 50-200 KDa
Exposure time：5s

Untransfected HEK-293F cells (green line) and transfected scFv-based Anti-CD19 CAR containing a Whitlow/218 linker stable cells (red line) were stained with anti-Whitlow linker antibody (2µg/1*10⁶cells), washed and then followed by FITC-conjugated anti-Mouse IgG Fc antibody and analyzed with flow cytometry.
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CUSABIO货号：CSB-MA234468A0m Whitlow linker单克隆抗体是针对人类Whitlow linker蛋白研发的高特异性科研试剂，适用于Western Blot、流式细胞术及ELISA等主流检测平台。该抗体经多维度验证可特异性识别Whitlow linker靶点，该蛋白作为细胞连接复合体的重要组分，在维持组织屏障完整性和细胞间通讯中发挥关键作用。本品采用杂交瘤技术制备，具备严格的批次一致性，在蛋白质印迹实验中能清晰呈现目标条带，流式检测中可实现精准的细胞表面标记分析。研究人员可将其应用于细胞黏附机制研究、上皮组织稳态调控、细胞信号转导通路解析等前沿领域，特别适用于探索Whitlow linker在细胞极性建立、创伤修复等生理过程中的分子机制。本产品为深入理解细胞连接相关蛋白网络提供可靠工具，是开展细胞生物学、分子互作及疾病机理研究的优选试剂，有效支持科研人员在蛋白质功能研究与分子通路探索中获得高质量实验数据。
基因名：

Whitlow linker
别名：

Whitlow/218 Linker (E3U7Q); Whitlow linker
宿主：

Mouse
反应种属：

Homo sapiens (Human)
免疫原：

Whitlow linker-KLH
标记方式：

Non-conjugated
克隆类型：

Monoclonal
抗体亚型：

IgG2b
纯化方式：

>95%, Protein G purified
克隆号：

18A3G5
浓度：

It differs from different batches. Please contact us to confirm it.
保存缓冲液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
产品提供形式：

Liquid
应用范围：

ELISA, WB, FC
推荐稀释比：

Application Recommended Dilution

WB WB：1:1000-1:5000

FC 1:50-1:200
Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Flow cytometry (FC) Protocol
储存条件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
货期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Whitlow linker Monoclonal Antibody

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Application	Recommended Dilution
WB	WB：1:1000-1:5000
FC	1:50-1:200