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  4. MCM9 Monoclonal Antibody

MCM9 Monoclonal Antibody

说明书
  • 中文名称：
    MCM9鼠单克隆抗体
  • 货号：
    CSB-MA882135A0m
  • 规格：
    ￥1320
  • 图片：
    • Western Blot
      Positive WB detected in: SY-5Y whole cell lysate, Hela whole cell lysate,U87 whole cell lysate
      All lanes: MCM9 antibody at 1:1000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 128, 73, 44, 85 kDa
      Observed band size: 128 kDa
      Exposure time：5min
    • IHC image of CSB-MA882135A0m diluted at 1:100 and staining in paraffin-embedded human testes tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • 其他：

产品详情

  • 产品描述：
    MCM9单克隆抗体（CUSABIO货号：CSB-MA882135A0m）是专为人源样本设计的科研试剂，针对DNA复制与修复关键调控因子MCM9蛋白进行精准检测。作为微染色体维持蛋白家族成员，MCM9在DNA解旋酶复合体组装及同源重组修复过程中发挥核心作用，其异常表达与基因组不稳定性密切相关。该抗体经严格验证适用于ELISA、Western Blot及免疫组化三大实验平台，具有优异的人源特异性反应能力。在基础研究领域，该产品可助力解析细胞周期调控机制，探索肿瘤发生发展过程中DNA复制应激的分子病理机制，并为生殖细胞发育、干细胞分化等生命过程的分子机理研究提供可靠工具。本产品采用杂交瘤技术制备，确保批次间高质量稳定性，适用于蛋白质相互作用研究、基因表达调控分析及疾病相关信号通路探索等前沿科研场景。通过特异性识别MCM9抗原表位，为研究人员在癌症生物学、遗传稳定性研究及抗肿瘤药物开发等领域提供高灵敏度的检测解决方案。
  • 产品名称：
    Mouse anti-Homo sapiens (Human) MCM9 Monoclonal Antibody antibody
  • Uniprot No.：
    Q9NXL9
  • 基因名：
    MCM9
  • 别名：
    MCM9 antibody; C6orf61 antibody; MCMDC1DNA helicase MCM9 antibody; hMCM9 antibody; EC 3.6.4.12 antibody; Mini-chromosome maintenance deficient domain-containing protein 1 antibody; Minichromosome maintenance 9 antibody
  • 宿主：
    Mouse
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human DNA helicase MCM9 protein (1-391AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal Antibody
  • 抗体亚型：
    IgG1
  • 纯化方式：
    >95%, Protein G purified
  • 克隆号：
    5H8E10
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:1000-1:5000
    IHC 1:50-1:200
  • Protocols：
    ELISA Protocol
    Western Blotting (WB) Protocol
    Immunohistochemistry (IHC) Protocol
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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MCM9 antibodies

MCM9 Proteins

靶点详情

  • 功能：
    Component of the MCM8-MCM9 complex, a complex involved in the repair of double-stranded DNA breaks (DBSs) and DNA interstrand cross-links (ICLs) by homologous recombination (HR). Required for DNA resection by the MRE11-RAD50-NBN/NBS1 (MRN) complex by recruiting the MRN complex to the repair site and by promoting the complex nuclease activity. Probably by regulating the localization of the MRN complex, indirectly regulates the recruitment of downstream effector RAD51 to DNA damage sites including DBSs and ICLs. Acts as a helicase in DNA mismatch repair (MMR) following DNA replication errors to unwind the mismatch containing DNA strand. In addition, recruits MLH1, a component of the MMR complex, to chromatin. The MCM8-MCM9 complex is dispensable for DNA replication and S phase progression. Probably by regulating HR, plays a key role during gametogenesis.
  • 基因功能参考文献：
    1. stalled replication forks can be restarted in S phase via homologous recombination using MCM8-9 as an alternative replicative helicase. PMID: 28487407
    2. Significant number of potentially damaging and novel variants in MCM9 in primary ovarian insufficiency; multiallelic association with variants in DDR and MCM8-MCM9 interactome genes. PMID: 27802094
    3. Study identified fifteen variants that included six common SNPs and nine variants of unknown significance (VUS) in MCM9 gene. However VUS occur in MCM9 in a small proportion of Lynch-like syndrome (LLS) patients and MCM9 mutations are unlikely to explain most LLS cases. PMID: 27886675
    4. Data show that the two affected sisters were homozygous for the mutation of MCM9 gene, encoding the minichromosome maintenance complex component 9. PMID: 26771056
    5. MCM9 loading onto chromatin is MSH2-dependent, and in turn MCM9 stimulates the recruitment of MLH1 to chromatin, revealing a role for MCM9 and its helicase activity in DNA mismatch repair. PMID: 26300262
    6. Autosomal-recessive variants in MCM9 cause a genomic-instability syndrome associated with hypergonadotropic hypogonadism and short stature. PMID: 25480036
    7. Chromatin immunoprecipitation analysis using human DR-GFP cells demonstrated that MCM8 and MCM9 proteins are rapidly recruited to DNA damage sites and promote RAD51 recruitment. PMID: 23401855
    8. A novel alternatively spliced variant of MCM9 is specifically induced after exposure to Mitomycin C. Expression is cell-cycle regulated and induced in S-phase. PMID: 23403237
    9. Identified a novel MCM family gene, MCM9, by using bioinformatics; mouse MCM9 mRNA was upregulated by transcription factor E2E1 and serum stimulation in NIH3T3 cells. PMID: 15850810
    10. We also show that the very recently reported human MCM9 protein (HsMCM9), which resembles a truncated MCM-like protein missing a part of the MCM2-7 signature domain, is an incomplete form of the full length HsMCM9 described here. PMID: 16226853
    11. Cdt1, with its two opposing regulatory binding factors MCM9 and geminin, appears to be a major platform on the pre-replication complexes to integrate cell-cycle signals. PMID: 18657502

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  • 相关疾病：
    Ovarian dysgenesis 4 (ODG4)
  • 亚细胞定位：
    Nucleus. Chromosome.
  • 蛋白家族：
    MCM family
  • 数据库链接：

    HGNC: 21484

    OMIM: 610098

    KEGG: hsa:254394

    STRING: 9606.ENSP00000314505

    UniGene: Hs.279008

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