Sheep tri-iodothyronine (T3) ELISA kit

产品详情

产品描述：

本试剂盒为绵羊三碘甲状腺原氨酸（T3）的定量检测而设计，适用于科研领域中对甲状腺激素代谢调控机制的研究。T3是甲状腺激素的活性形式，参与调节基础代谢率、能量平衡及生长发育等关键生理过程，其水平变化可反映甲状腺功能状态或相关代谢疾病模型的动态特征。本产品采用竞争性酶联免疫分析法，可精准检测血清、血浆、细胞培养上清液及组织匀浆中的T3含量，检测灵敏度为0.5 ng/mL，线性范围覆盖0.5-8 ng/mL，能够满足不同样本类型的浓度检测需求。试剂盒包含预包被抗体及优化反应体系，操作流程标准化，适用于动物生理学研究中甲状腺功能评估、内分泌干扰物效应分析、药物干预实验中的激素水平监测，或体外细胞模型中甲状腺激素分泌机制研究。科研人员可通过该试剂盒高效获取实验数据，为探究甲状腺相关代谢通路、疾病模型构建及药理学研究提供可靠工具，但不可用于临床诊断或治疗监测。
缩写：

T3
种属：

Ovis aries (Sheep)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

0.5 ng/mL-8 ng/mL
灵敏度：

0.5 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of sheepT3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	90-100
1:2	Average %	87
	Range %	85-96
1:4	Average %	95
	Range %	90-100
1:8	Average %	95
	Range %	82-99

回收率：

The recovery of sheep T3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	85-102
EDTA plasma (n=4)	96	90-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.