Sheep neutrophil gelatinase-associated lipocalin,NGAL ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO绵羊中性粒细胞明胶酶相关脂质运载蛋白（NGAL）酶联免疫检测试剂盒（货号：CSB-E13392Sh），采用竞争法原理实现定量检测，适用于血清、血浆及组织匀浆样本中NGAL蛋白的科研分析。NGAL作为急性肾损伤和炎症反应的重要生物标志物，在绵羊疾病模型研究中具有重要价值。该试剂盒检测灵敏度达15.6 pg/mL，线性范围覆盖15.6-1000 pg/mL，通过预包被特异性抗体实现样本中NGAL与酶标物的竞争性结合，结合显色反应后采用450nm波长测定吸光度值，3小时内即可完成检测流程。产品包含标准品、检测抗体、底物等完整组分，适用于绵羊炎症机制研究、肾脏疾病动物模型建立、药物干预效果评价等科研领域。其高灵敏特性可有效检测低丰度样本，重复实验显示批内批间稳定性良好，为实验室开展NGAL相关病理生理研究提供可靠工具。
缩写：

NGAL
种属：

Ovis aries (Sheep)
样本类型：

serum, plasma, tissue homogenates
检测范围：

15.6 pg/mL-1000 pg/mL
灵敏度：

3.9 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of sheep NGAL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	92
	Range %	86-95
1:400	Average %	103
	Range %	99-105
1:800	Average %	94
	Range %	88-98
1:1600	Average %	99
	Range %	94-105

回收率：

The recovery of sheep NGAL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	88-97
EDTA plasma (n=4)	95	88-99

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.