Sheep Interleukin 2,IL-2 ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CSB-E11217Sh绵羊白介素2(IL-2)酶联免疫试剂盒采用双抗体夹心法原理，专为科研领域定量检测绵羊血清、血浆及组织匀浆样本中的IL-2水平而设计。IL-2是一种主要由活化的T淋巴细胞分泌的细胞因子，在调节免疫应答、促进T细胞增殖及维持免疫稳态中发挥核心作用，其表达水平常被用于评估机体的免疫活性状态。本试剂盒检测灵敏度高，线性范围为31.25-2000 pg/mL，可精准覆盖生理及实验条件下IL-2的浓度变化。实验操作需使用50-100μL样本，通过预包被的捕获抗体与样本中IL-2特异性结合，经酶标检测抗体显色后，利用标准曲线实现定量分析。该产品适用于动物免疫学研究、疫苗效力评估、炎症反应模型构建等科研场景，为探究绵羊免疫系统功能、感染性疾病机制或药物干预效果提供可靠数据支持。所有组分经严格验证，确保批间稳定性和特异性，实验全程无需特殊设备，兼容常规酶标仪分析。
别名：

IL2Interleukin-2 ELISA kit; IL-2 ELISA kit; T-cell growth factor ELISA kit; TCGF ELISA kit
缩写：

IL2
Uniprot No.：

P19114
种属：

Ovis aries (Sheep)
样本类型：

serum, plasma, tissue homogenates
检测范围：

31.25 pg/mL-2000 pg/mL
灵敏度：

7.81 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of sheep IL-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	84-93
1:2	Average %	98
	Range %	91-105
1:4	Average %	97
	Range %	95-103
1:8	Average %	92
	Range %	86-98

回收率：

The recovery of sheep IL-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-99
EDTA plasma (n=4)	96	90-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
亚细胞定位：

Secreted.
蛋白家族：

IL-2 family
数据库链接：

KEGG: oas:443401

UniGene: Oar.436