Sheep Interleukin 12,IL-12 ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO试剂盒采用双抗体夹心酶联免疫吸附法（ELISA）定量检测绵羊血清、血浆及组织匀浆样本中的白介素12（IL-12），其检测灵敏度覆盖31.25至2000 pg/mL的宽线性范围。IL-12作为重要的促炎性细胞因子，在固有免疫和适应性免疫中发挥核心调控作用，主要由树突状细胞和巨噬细胞分泌，通过激活T细胞与NK细胞促进γ干扰素（IFN-γ）产生，参与Th1型免疫应答通路。该检测体系基于预包被于微孔板的高特异性捕获抗体，可定向结合样本中的IL-12抗原，随后通过生物素标记的检测抗体形成"三明治"复合物，最终借助链霉亲和素-HRP酶联系统进行信号放大，显色强度与目标物浓度呈正相关。本产品适用于绵羊免疫相关疾病的机制研究，如感染性疾病模型中细胞免疫应答强度的动态监测、疫苗效力评估实验中的Th1型免疫激活分析，或炎症性疾病研究中的细胞因子网络调控解析，为兽医科学及比较医学研究提供精准的科研工具。
别名：

N/A
缩写：

IL-12
种属：

Ovis aries (Sheep)
样本类型：

serum, plasma, tissue homogenates
检测范围：

31.25 pg/mL-2000 pg/mL
灵敏度：

7.81 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of sheep IL-12 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	87-94
1:2	Average %	103
	Range %	99-107
1:4	Average %	93
	Range %	87-99
1:8	Average %	91
	Range %	88-96

回收率：

The recovery of sheep IL-12 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	92-103
EDTA plasma (n=4)	90	86-94

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.