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  4. Sheep Interferon γ,IFN-γ ELISA Kit

Sheep Interferon γ,IFN-γ ELISA Kit

文献引用 (5) 说明书下载
  • 中文名称：
    绵羊γ干扰素(IFN-γ)酶联免疫试剂盒
  • 货号：
    CSB-E14018Sh
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO绵羊γ干扰素（IFN-γ）酶联免疫检测试剂盒（货号：CSB-E14018Sh），采用双抗体夹心法定量检测绵羊血清、血浆或组织匀浆样本中的IFN-γ含量，检测灵敏度为5 pg/mL，线性范围为5-200 pg/mL，适用于科研场景下对免疫相关机制的探索。IFN-γ是由T淋巴细胞和自然杀伤细胞分泌的关键细胞因子，在抗病毒、抗胞内病原体感染及肿瘤免疫监视中发挥核心作用，其表达水平可反映机体的细胞免疫应答强度。本试剂盒通过特异性捕获抗体和检测抗体的协同作用实现目标蛋白的高精度定量，可用于评估绵羊在疫苗接种、寄生虫感染（如旋毛虫病）或细菌性疾病（如布鲁氏菌病）等实验模型中Th1型免疫反应的动态变化，亦适用于比较不同处理条件下免疫调节机制的研究。该试剂盒操作便捷，支持多种生物样本类型分析，为畜牧科学、兽医学及比较免疫学领域的研究者提供可靠的检测工具。
  • 别名：
    IFNG ELISA Kit; Interferon gamma ELISA Kit; IFN-gamma ELISA Kit
  • 缩写：
    IFNG
  • Uniprot No.：
  • 种属：
    Ovis aries (Sheep)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    5 pg/mL-200 pg/mL
  • 灵敏度：
    2.5 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of sheep IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 98  
    Range % 95-102  
    1:2 Average % 87  
    Range % 84-90  
    1:4 Average % 85  
    Range % 82-89  
    1:8 Average % 93  
    Range % 90-96  
  • 回收率：
    The recovery of sheep IFN-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 92 87-95  
    EDTA plasma (n=4) 86 83-89  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    200 2.776 2.798 2.787    
    100 2.088 2.098 2.093    
    50 1.164 1.187 1.176    
    25 0.675 0.633 0.654    
    10 0.264 0.287 0.276    
    5 0.125 0.127 0.126    
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

引用文献

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IFNG Proteins

靶点详情

  • 功能：
    Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation. Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation. Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription. Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits. In turn, increases the quantity, quality, and repertoire of peptides for class I MHC loading. Increases the efficiency of peptide generation also by inducing the expression of activator PA28 that associates with the proteasome and alters its proteolytic cleavage preference. Up-regulates as well MHC II complexes on the cell surface by promoting expression of several key molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL. Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation.
  • 基因功能参考文献：
    1. gammadelta T cells traveling in the skin-draining afferent lymph are effectors that produce IFN-gamma or IL-17 and express high levels of the skin- and inflammation-seeking molecule E-selectin ligand PMID: 23838472
    2. structural and functional characterization and role in haemonchiasis resistance PMID: 21419496
    3. results suggest that intron 1 of the interferon gamma gene has an important role in resistance to nematode infection in the Texel breed PMID: 16054889
    4. Five unique polymorphic patterns were detected and sequence analysis revealed that these patterns represent five different IFNG alleles. PMID: 16820277
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Type II (or gamma) interferon family
  • 组织特异性：
    Released primarily from activated T lymphocytes.
  • 数据库链接：

    KEGG: oas:443396

    UniGene: Oar.457

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九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |