Sheep C-reactive protein(CRP) ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO绵羊C反应蛋白(CRP)酶联免疫检测试剂盒（货号CSB-E15081Sh），采用双抗体夹心法定量检测血清、血浆、组织匀浆及细胞裂解液中的CRP蛋白。CRP是一种由肝脏合成的急性期反应蛋白，在炎症、感染或组织损伤时显著升高，可作为评估机体免疫应答和炎症状态的重要生物标志物。本试剂盒检测范围覆盖0.625至40 μg/mL，通过预包被特异性捕获抗体与样本中的CRP结合，再经检测抗体与酶标记物放大信号，最终实现目标蛋白的精准定量分析。适用于绵羊模型中炎症机制研究、疾病进程评估及免疫调控相关科研实验，例如感染性疾病模型构建中CRP动态监测、应激反应评估或药物干预效果分析。其宽泛的样本兼容性支持多种实验设计需求，操作流程标准化，重复性良好，可为炎症相关基础研究提供可靠数据支持。
缩写：

CRP
Uniprot No.：

W5PD71
种属：

Ovis aries (Sheep)
样本类型：

serum, plasma, tissue homogenates, cell lysates
检测范围：

0.625 μg/mL-40 μg/mL
灵敏度：

0.156 μg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of sheep CRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	80-90
1:2	Average %	96
	Range %	92-100
1:4	Average %	92
	Range %	87-96
1:8	Average %	95
	Range %	87-98

回收率：

The recovery of sheep CRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-96
EDTA plasma (n=4)	90	84-95

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.