Rat β2-microglobulin,BMG/β2-MG ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO大鼠β2微球蛋白（BMG/β2-MG）酶联免疫检测试剂盒（货号：CSB-E11298r），采用夹心法原理实现定量分析，适用于血清、血浆、尿液及组织匀浆样本中β2-MG含量的精确测定。β2微球蛋白是主要组织相容性复合体I类分子（MHC-I）的恒定链成分，广泛存在于细胞表面，其血清浓度与肾小球滤过功能及免疫系统状态密切相关，在肾脏疾病、免疫调节及肿瘤微环境研究中具有重要科研价值。本试剂盒检测灵敏度达0.156 μg/mL，线性范围覆盖0.156-10 μg/mL，可稳定检测不同生物样本中的低丰度β2-MG。实验方案支持96孔板操作，兼容常规实验室设备，适用于探索肾脏生理病理机制、评估药物肾毒性、肿瘤免疫微环境分析等基础研究领域，尤其为尿液样本的肾小管功能研究提供可靠工具。试剂盒包含预包被抗体、标准品及检测所需全套组分，提供标准曲线建立与样本浓度计算的完整解决方案。
别名：

B2MBeta-2-microglobulin ELISA kit
缩写：

B2M
Uniprot No.：

P01885
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, urine, tissue homogenates
检测范围：

0.156 μg/mL-10 μg/mL
灵敏度：

0.039 μg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat BMG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	82-92
1:2	Average %	96
	Range %	92-101
1:4	Average %	100
	Range %	95-105
1:8	Average %	90
	Range %	85-95

回收率：

The recovery of rat BMG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	90-104
EDTA plasma (n=4)	93	89-97

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system.
亚细胞定位：

Secreted.
蛋白家族：

Beta-2-microglobulin family
数据库链接：

STRING: 9986.ENSOCUP00000014707