Rat α2 macroglobulin,α2 MG ELISA Kit

产品详情

产品描述：

CUSABIO大鼠α2巨球蛋白（α2-MG）酶联免疫检测试剂盒（货号CSB-E13863r），基于竞争法定量原理开发，适用于血清、血浆样本中α2-MG的精准检测。α2-MG是一种广谱蛋白酶抑制剂，在炎症反应调控、组织修复及免疫调节中发挥重要作用，其表达水平与肝损伤、肾病及代谢性疾病的发生发展密切相关。本试剂盒检测范围为3.13 ng/mL至800 ng/mL，灵敏度高，可覆盖生理及病理条件下样本的检测需求。试剂盒包含预包被酶标板、标准品、检测抗体及显色底物等核心组分，操作流程简便，仅需样本预处理、孵育结合、洗涤和显色读数等步骤即可完成检测。建议短期保存于4℃（3个月内使用），长期保存需置于-20℃，避免反复冻融以确保试剂活性。该产品专为科研领域设计，可应用于炎症机制研究、肝肾功能评估、疾病动物模型分析及药物干预效果评价等方向，为探索α2-MG在病理生理过程中的作用提供可靠工具。
别名：

A2m ELISA kit; A2m1Alpha-2-macroglobulin ELISA kit; Alpha-2-M ELISA kit
缩写：

A2M
Uniprot No.：

P06238
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma
检测范围：

3.13 ng/mL-800 ng/mL
灵敏度：

3.125 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat α2 MG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	97
	Range %	88-105
1:2000	Average %	91
	Range %	89-102
1:4000	Average %	93
	Range %	90-98
1:8000	Average %	94
	Range %	87-98

回收率：

The recovery of rat α2 MG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	92-102
EDTA plasma (n=4)	95	90-99

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
亚细胞定位：

Secreted.
蛋白家族：

Protease inhibitor I39 (alpha-2-macroglobulin) family
组织特异性：

Highest constitutive expression in ovary. Low level in testis, uterus and non-acute phase liver. Protein found in plasma.
数据库链接：

KEGG: rno:100911545

STRING: 10116.ENSRNOP00000019346

UniGene: Rn.225884