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  4. Rat α-enolase ELISA Kit

Rat α-enolase ELISA Kit

说明书下载
  • 中文名称：
    大鼠α烯醇化酶(α-enolase)酶联免疫试剂盒
  • 货号：
    CSB-E17439r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO专为大鼠样本设计的α烯醇化酶（α-enolase）定量检测试剂盒（货号：CSB-E17439r），采用夹心法酶联免疫吸附原理，可灵敏检测血清、血浆及组织匀浆样本中0.625 ng/mL至40 ng/mL浓度范围内的α-烯醇化酶含量。α-烯醇化酶作为糖酵解途径的关键酶，广泛参与细胞能量代谢调控，其表达水平与细胞增殖、分化及多种病理过程密切相关，在代谢性疾病、肿瘤微环境及炎症反应等研究领域具有重要价值。试剂盒提供优化的预包被抗体及标准品体系，支持多样本类型的重复性检测，适用于科研场景下对大鼠模型代谢通路异常、疾病机制探索或药物干预效果评估等实验需求，操作流程标准化且兼容常规酶标仪读值，为生命科学基础研究提供可靠工具。
  • 别名：
    Eno1 ELISA Kit; Eno-1Alpha-enolase ELISA Kit; EC 4.2.1.11 ELISA Kit; 2-phospho-D-glycerate hydro-lyase ELISA Kit; Enolase 1 ELISA Kit; Non-neural enolase ELISA Kit; NNE ELISA Kit
  • 缩写：
    ENO1
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.625 ng/mL-40 ng/mL
  • 灵敏度：
    0.156 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat α-enolase in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 93
    Range % 88-97
    1:2 Average % 102
    Range % 97-107
    1:4 Average % 101
    Range % 95-105
    1:8 Average % 94
    Range % 91-98
  • 回收率：
    The recovery of rat α-enolase spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 105 99-108
    EDTA plasma (n=4) 87 84-92
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    40 2.191 2.275 2.233 2.111
    20 1.448 1.538 1.493 1.371
    10 0.921 0.963 0.942 0.820
    5 0.563 0.572 0.568 0.446
    2.5 0.334 0.341 0.338 0.216
    1.25 0.231 0.245 0.238 0.116
    0.625 0.173 0.179 0.176 0.054
    0 0.121 0.122 0.122 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

产品评价

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Eno1 Proteins

靶点详情

  • 功能：
    Glycolytic enzyme the catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate. In addition to glycolysis, involved in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.
  • 基因功能参考文献：
    1. gamma-enolase may signal and correlate lung tissue damage well before obvious physical pathological tissue damage and might be a candidate diagnostic and/or prognostic marker. PMID: 29728894
    2. The present study aimed to investigate the change of alpha-enolase during cardiac hypertrophy and explore its role in this pathological process. PMID: 28910549
    3. alpha-enolase has a catalytically independent role in inducing cardiomyocyte apoptosis and mitochondrial dysfunction. PMID: 25446184
    4. this research suggests that the protective effect of Hsc70 on the cardiomyocytes against oxidative stress is partly associated with its interaction with alpha-enolase. PMID: 21958194
    5. alpha-Enolase binds to RNA PMID: 21621582
    6. The alpha-enolase hyper-phosphorylation in hypertrophied left ventricle was accompanied by decreased enzymatic activity. PMID: 18468517
    7. Data show that the expression of alpha-enolase in the left ventricle of the rats during normal and pathological cardiac development is different. PMID: 19450578
    8. enolase alpha was up-regulated in left ventricle of spontaneously hypertensive rats. PMID: 16485256

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  • 亚细胞定位：
    Cytoplasm. Cell membrane. Note=Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form.
  • 蛋白家族：
    Enolase family
  • 组织特异性：
    Expressed in flagella of epididymal sperm. The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma
  • 数据库链接：

    KEGG: rno:24333

    STRING: 10116.ENSRNOP00000024106

    UniGene: Rn.117044

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九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |