Rat urokinase plasminogen activator,uPA ELISA kit

中文名称：

大鼠尿激酶型纤溶酶原激活物(uPA)酶联免疫试剂盒
货号：

CSB-E07368r
规格：

96T/48T
价格：

￥3900/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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CUSABIOCSB-E07368r大鼠尿激酶型纤溶酶原激活物(uPA)酶联免疫检测试剂盒，采用双抗体夹心法定量检测大鼠血清、血浆或组织匀浆中的uPA浓度。uPA作为丝氨酸蛋白酶家族成员，通过激活纤溶酶原参与细胞外基质降解，在组织重塑、血管生成及肿瘤转移等生理病理过程中发挥重要作用。试剂盒检测灵敏度达15.6 pg/mL，线性范围为15.6-1000 pg/mL，标准曲线涵盖7个梯度浓度点，通过预包被特异性捕获抗体与标记检测抗体的协同作用实现精准定量。实验流程包含样本预处理、孵育反应及显色测定步骤，操作时间约3小时，配套提供标准品、检测抗体及终止液等完整组分。适用于心血管疾病、纤维化疾病或肿瘤相关动物模型中纤溶系统活性的动态监测，以及细胞迁移、组织修复等基础研究中的uPA表达水平分析，为科研人员探究uPA在病理生理过程中的调控机制提供可靠工具。
别名：

PlauUrokinase-type plasminogen activator ELISA kit; U-plasminogen activator ELISA kit; uPA ELISA kit; EC 3.4.21.73) [Cleaved into: Urokinase-type plasminogen activator long chain A; Urokinase-type plasminogen activator short chain A; Urokinase-type plasminogen activator chain B] ELISA kit
缩写：

PLAU
Uniprot No.：

P29598
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

15.6 pg/mL-1000 pg/mL
灵敏度：

3.9 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat uPA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	88-95
1:2	Average %	95
	Range %	90-100
1:4	Average %	97
	Range %	94-103
1:8	Average %	101
	Range %	96-105

回收率：

The recovery of rat uPA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-94
EDTA plasma (n=4)	96	94-101

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.334	2.186	2.260	2.077
500	1.652	1.625	1.639	1.456
250	1.047	0.997	1.022	0.839
125	0.636	0.688	0.662	0.479
62.5	0.398	0.402	0.400	0.217
31.2	0.325	0.337	0.331	0.148
15.6	0.246	0.232	0.239	0.056
0	0.189	0.177	0.183

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

引用文献

In vivo evaluation of urokinase-loaded hollow nanogels forsonothrombolysis on suture embolization-induced acute ischemicstroke rat model Yuming Teng.et al,Bioactive Materials,2017
In vivo evaluation of urokinase-loaded hollow nanogels for sonothrombolysis on suture embolization-induced acute ischemic stroke rat model YumingTeng.et al,Bioactive Materials,2017

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功能：

Specifically cleaves the zymogen plasminogen to form the active enzyme plasmin.
亚细胞定位：

Secreted.
蛋白家族：

Peptidase S1 family
数据库链接：

STRING: 10116.ENSRNOP00000014273

UniGene: Rn.6064

Rat urokinase plasminogen activator,uPA ELISA kit

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