Rat typeⅠ procollagen,PC I ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

大鼠Ⅰ型前胶原(PCⅠ)酶联免疫试剂盒（CSB-E11249r）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液样本中的PCⅠ含量。试剂盒检测范围为62.5 pg/mL-4000pg/mL，可为研究肝纤维化模型、皮肤再生机制或骨基质代谢等科研领域提供可靠工具，适用于体外实验中对生物样本中PCⅠ蛋白水平的精确量化分析，为科研人员探索相关分子机制提供高效解决方案。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

type 1 procollagen,PC I,_x000D_ type 1 procollagen,PC I
缩写：

PCⅠ
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, cell culture supernates
检测范围：

62.5 pg/mL-4000pg/mL
灵敏度：

15.6 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat PCⅠ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	93
	Range %	90-96
1:10	Average %	87
	Range %	84-90
1:20	Average %	84
	Range %	81-87
1:40	Average %	92
	Range %	88-96

回收率：

The recovery of rat PCⅠ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	100	97-103
EDTA plasma (n=4)	85	82-89

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.