Rat tissue factor(TF)/coagulation factor VII(F7) complex ELISA kit

产品详情

产品描述：

大鼠组织因子与活化凝血因子7复合物酶联免疫试剂盒（CSB-EQ027252RA）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TF含量。TF即组织因子，是一种跨膜糖蛋白。它是外源性凝血途径的启动因子，在生理止血中起关键作用。研究发现TF还参与肿瘤生长、转移及血管生成等。其作用机制主要是通过与凝血因子结合，启动凝血级联反应并激活相关信号通路。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于心血管疾病、血栓性疾病等动物模型的机制研究，或抗凝药物、靶向治疗方案的体外效果评估。为凝血相关基础研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

TF
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

15.6 pg/mL-1000 pg/mL
灵敏度：

3.9 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat TF/F7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	107
	Range %	98-110
1:2	Average %	103
	Range %	96-107
1:4	Average %	98
	Range %	89-102
1:8	Average %	105
	Range %	98-110

回收率：

The recovery of rat TF/F7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	85-96
EDTA plasma (n=4)	102	95-107

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.