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Rat tight junction protein 1 (ZO1) ELISA Kit

  • 中文名称:
    大鼠紧密连接蛋白1(ZO1)酶联免疫试剂盒
  • 货号:
    CSB-E17287r
  • 规格:
    96T/48T
  • 价格:
    ¥3600/¥2500
  • 其他:

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  • 产品描述:
    大鼠紧密连接蛋白1(ZO1)酶联免疫试剂盒(CSB-E17287r)为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆、细胞裂解物样本中的ZO1含量。大鼠紧密连接蛋白1(ZO-1)是紧密连接复合体的重要组成部分,参与维持上皮细胞间连接的稳定性和肠道屏障功能。它通过连接膜蛋白与细胞骨架,调节细胞间通透性,对防止病原体和毒素进入体内至关重要。研究显示,ZO-1的表达与消化系统疾病的发生、发展及预后密切相关,其功能异常可能导致肠黏膜屏障破坏和多种消化系统疾病。试剂盒检测范围为31.25 pg/mL-2000 pg/mL,适用于体外研究ZO1在细胞模型或动物模型中的表达调控,例如药物干预、氧化应激或病原体感染等实验条件下紧密连接功能的动态评估,为屏障相关疾病的分子机制研究提供可靠工具本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 别名:
    DKFZp686M05161, MGC133289, ZO-1, tight junction protein 1|tight junction protein ZO-1|zona occludens 1|zonula occludens 1 protein
  • 缩写:
    ZO1
  • 种属:
    Rattus norvegicus (Rat)
  • 样本类型:
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围:
    31.25 pg/mL-2000 pg/mL
  • 灵敏度:
    7.8 pg/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Tags & Cell Markers
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rat ZO1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 91  
    Range % 86-95  
    1:2 Average % 102  
    Range % 97-107  
    1:4 Average % 91  
    Range % 85-97  
    1:8 Average % 97  
    Range % 91-103  
  • 回收率:
    The recovery of rat ZO1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 89-98  
    EDTA plasma (n=4) 97 90-100  
                 
                 
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    2000 2.272 2.374 2.323 2.221  
    1000 1.846 1.967 1.907 1.805  
    500 1.496 1.392 1.444 1.342  
    250 0.962 0.946 0.954 0.852  
    125 0.578 0.596 0.587 0.485  
    62.5 0.364 0.351 0.358 0.256  
    31.2 0.225 0.230 0.228 0.126  
    0 0.101 0.102 0.102    
  • 数据处理:
  • 货期:
    3-5 working days

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