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中文名称:大鼠紧密连接蛋白1(ZO1)酶联免疫试剂盒
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货号:CSB-E17287r
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规格:96T/48T
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价格:¥3600/¥2500
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其他:
产品详情
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产品描述:大鼠紧密连接蛋白1(ZO1)酶联免疫试剂盒(CSB-E17287r)为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆、细胞裂解物样本中的ZO1含量。大鼠紧密连接蛋白1(ZO-1)是紧密连接复合体的重要组成部分,参与维持上皮细胞间连接的稳定性和肠道屏障功能。它通过连接膜蛋白与细胞骨架,调节细胞间通透性,对防止病原体和毒素进入体内至关重要。研究显示,ZO-1的表达与消化系统疾病的发生、发展及预后密切相关,其功能异常可能导致肠黏膜屏障破坏和多种消化系统疾病。试剂盒检测范围为31.25 pg/mL-2000 pg/mL,适用于体外研究ZO1在细胞模型或动物模型中的表达调控,例如药物干预、氧化应激或病原体感染等实验条件下紧密连接功能的动态评估,为屏障相关疾病的分子机制研究提供可靠工具本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
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别名:DKFZp686M05161, MGC133289, ZO-1, tight junction protein 1|tight junction protein ZO-1|zona occludens 1|zonula occludens 1 protein
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缩写:ZO1
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种属:Rattus norvegicus (Rat)
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样本类型:serum, plasma, tissue homogenates, cell lysates
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检测范围:31.25 pg/mL-2000 pg/mL
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灵敏度:7.8 pg/mL
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反应时间:1-5h
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样本体积:50-100ul
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检测波长:450 nm
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研究领域:Tags & Cell Markers
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测定原理:quantitative
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测定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of rat ZO1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 91 Range % 86-95 1:2 Average % 102 Range % 97-107 1:4 Average % 91 Range % 85-97 1:8 Average % 97 Range % 91-103 -
回收率:
The recovery of rat ZO1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 95 89-98 EDTA plasma (n=4) 97 90-100 -
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average Corrected 2000 2.272 2.374 2.323 2.221 1000 1.846 1.967 1.907 1.805 500 1.496 1.392 1.444 1.342 250 0.962 0.946 0.954 0.852 125 0.578 0.596 0.587 0.485 62.5 0.364 0.351 0.358 0.256 31.2 0.225 0.230 0.228 0.126 0 0.101 0.102 0.102 -
数据处理:
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货期:3-5 working days
引用文献
- Microbiota-derived tryptophan metabolism and AMPK/mTOR pathway mediate antidepressant-like effect of Shugan Hewei Decoction Y Yue, Y Ke, J Zheng, Z Wang, H Liu,Frontiers in Pharmacology,2024
- Activation of AMPK/SIRT1/FOXO3a signaling by BMS-477118 (saxagliptin) mitigates chronic colitis in rats: uncovering new anti-inflammatory and antifibrotic roles EA Elmorsy, ME Youssef, MR Abdel-Hamed,Frontiers in Pharmacology,2024
- Rice bran extract mitigates depressive-like behavior in dextran sulfate sodium-induced colitis: Involvement of the gut-brain axis and Sirt1 signaling pathway HM Shendy,Food and chemical toxicology,2023
- Niacin modulates depressive-like behavior in experimental colitis through GPR109A-dependent mechanisms W Wadie,Life sciences,2023
- (R, R)-BD-AcAc2 Mitigates Chronic Colitis in Rats: A Promising Multi-Pronged Approach Modulating Inflammasome Activity, Autophagy, and Pyroptosis S Saber,Pharmaceuticals,2023
- Gut microbiota-derived melatonin from Puerariae Lobatae Radix-resistant starch supplementation attenuates ischemic stroke injury via a positive microbial co-occurrence pattern Z Lian,Pharmacological research,2023
- Potentilla chinensis aqueous extract attenuates cyclophosphamide-induced hemorrhagic cystitis in rat model K Juszczak,Scientific Reports,2022
- The influence of Potentilla chinensis aqueous extract on urinary bladder function in retinyl acetate-induced detrusor overactivity in rats A Wróbel,Biomedicine & Pharmacotherapy,2020
- Puerariae Lobatae Radix with chuanxiong Rhizoma for treatment of cerebral ischemic stroke by remodeling gut microbiota to regulate the brain-gut barriers RunzhiChen, et al,Journal of nutritional biochemistry,2018
- The combination of Puerariae Lobatae Radix and Chuanxiong Rhizoma enhanced the absorption and pharmacokinetics of puerarin by modulating the intestinal barrier and influenced gut microbiota Runzhi Chen.et al,Journal of Functional Foods,2018
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