Rat tartrate-resistant acid phosphatase 5b,TRACP-5b ELISA Kit

Rat tartrate-resistant acid phosphatase 5b,TRACP-5b ELISA Kit

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中文名称：

大鼠抗酒石酸酸性磷酸酶5b(TRACP-5b)酶联免疫试剂盒
货号：

CSB-E08491r
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO大鼠抗酒石酸酸性磷酸酶5b（TRACP-5b）酶联免疫检测试剂盒（货号：CSB-E08491r），基于双抗体夹心法原理实现定量分析，适用于检测大鼠血清、血浆及组织匀浆样本中的TRACP-5b水平。TRACP-5b是由破骨细胞特异性分泌的蛋白酶，其活性与骨吸收过程密切相关，是评估骨代谢动态平衡的重要生物标志物，在骨质疏松机制研究、骨骼疾病模型评估及骨代谢相关药物开发等领域具有重要科研价值。试剂盒检测灵敏度高，线性范围为0.312 mIU/mL至20 mIU/mL，可精准检测微量样本中目标蛋白的浓度变化。实验操作流程标准化，配备完整实验组分，适用于体外定量分析骨代谢相关动物模型的生物标志物表达水平，为骨生物学研究、药物干预效果评价及病理机制探索提供可靠工具。本产品严格遵循科研用途设计，实验结果需结合具体实验条件进行生物学意义解析。
缩写：

TRACP-5b
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.312 mIU/mL-20 mIU/mL
灵敏度：

0.078 mIU/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat TRACP-5b in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	89-95
1:2	Average %	100
	Range %	97-103
1:4	Average %	93
	Range %	88-97
1:8	Average %	98
	Range %	93-102

回收率：

The recovery of rat TRACP-5b spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-96
EDTA plasma (n=4)	95	91-97

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

mIU/ml	OD1	OD2	Average	Corrected
20	2.076	1.988	2.032	1.887
10	1.292	1.196	1.244	1.099
5	0.804	0.820	0.812	0.667
2.5	0.511	0.521	0.516	0.371
1.25	0.324	0.335	0.330	0.185
0.625	0.282	0.278	0.280	0.135
0.312	0.223	0.212	0.218	0.073
0	0.146	0.144	0.145

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.