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  4. Rat soluble platelet endothelial cell adhesion molecule 1,sPECAM-1 ELISA Kit

Rat soluble platelet endothelial cell adhesion molecule 1,sPECAM-1 ELISA Kit

文献引用 (2) 说明书下载
  • 中文名称：
    大鼠可溶性血小板内皮细胞粘附分子1(sPECAM-1/sCD31)酶联免疫试剂盒
  • 货号：
    CSB-E08134r
  • 规格：
    96T/48T
  • 价格：
    ￥3900/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO定量检测大鼠血清、血浆及组织匀浆样本中可溶性血小板内皮细胞粘附分子1（sPECAM-1/sCD31）的酶联免疫试剂盒，采用双抗体夹心法原理进行检测。sPECAM-1作为免疫球蛋白超家族成员，广泛表达于血管内皮细胞和血小板表面，参与血管通透性调节、炎症反应及白细胞跨内皮迁移等生理病理过程，是研究血管相关疾病机制的重要分子标志物。试剂盒检测灵敏度达3.12 ng/mL，线性检测范围为3.12-200 ng/mL，可稳定检测包括组织匀浆在内的多种样本类型，适用于心血管疾病动物模型分析、炎症机制研究以及药物干预效果评价等科研领域。通过预包被捕获抗体与检测抗体的特异性结合，配合显色系统实现目标蛋白的精准定量，为探索血管内皮功能异常相关疾病提供可靠的实验工具。
  • 别名：
    Pecam1 ELISA Kit; Pecam ELISA Kit; Platelet endothelial cell adhesion molecule ELISA Kit; PECAM-1 ELISA Kit; CD antigen CD31 ELISA Kit
  • 缩写：
    PECAM1
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    3.12 ng/mL-200 ng/mL
  • 灵敏度：
    0.78 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat sPECAM-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 92    
    Range % 86-95    
    1:2 Average % 102    
    Range % 97-107    
    1:4 Average % 91    
    Range % 85-97    
    1:8 Average % 97    
    Range % 91-103    
  • 回收率：
    The recovery of rat sPECAM-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 95 89-98    
    EDTA plasma (n=4) 97 90-100    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    ng/ml OD1 OD2 Average Corrected    
    200 2.468 2.554 2.511 2.372    
    100 1.656 1.732 1.694 1.555    
    50 1.033 1.067 1.050 0.911    
    25 0.591 0.611 0.601 0.462    
    12.5 0.380 0.398 0.389 0.250    
    6.25 0.278 0.286 0.282 0.143    
    3.12 0.203 0.207 0.205 0.066    
    0 0.138 0.139 0.139 　    
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

引用文献

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Pecam1 Proteins

靶点详情

  • 功能：
    Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-660 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions. Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils. Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal. Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes. Modulates bradykinin receptor BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells. Induces susceptibility to atherosclerosis.
  • 基因功能参考文献：
    1. Circulating PECAM-1 is a promising biomarker for the detection and monitoring of ventilator-induced lung injury. PMID: 24588994
    2. Shock wave therapy leads to mobilization of endogenous CD31/CD34-positive endothelial cells. PMID: 23395097
    3. PECAM-1 expression on PMNs is in a converse way between pancreatic microcirculation and peripheral circulation in acute edematous pancreatitis PMID: 14599961
    4. A reverse expression of PECAM-1 on neutrophils was found between peripheral circulation and pancreatic microcirculation, so inhibition of PECAM-1 expression may improve the pathological change of acute edematous pancreatitis. PMID: 15655817
    5. expression pattern of PECAM & VE-cadherin at endothelial cell junctions in adult initial lymphatic network; results suggest that primary valves are associated with discontinuous expression of endothelial junction molecules PMID: 17935476

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  • 亚细胞定位：
    Cell membrane; Single-pass type I membrane protein. Membrane raft. Cell junction.
  • 数据库链接：

    KEGG: rno:29583

    STRING: 10116.ENSRNOP00000044403

    UniGene: Rn.1878

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