Rat soluble E-selectin,sE-selectin ELISA Kit

Rat soluble E-selectin,sE-selectin ELISA Kit

产品详情

引用文献

产品评价

靶点详情

产品详情

引用文献

产品评价

靶点详情

Most popular with customers

产品中心

信号通路

技术信息

联系信息

中文名称：

大鼠可溶性E选择素(sE-selectin)酶联免疫试剂盒
货号：

CSB-E07996r
规格：

96T/48T
价格：

￥3900/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情
产品评价
引用文献
靶点详情

产品描述：

CUSABIO大鼠可溶性E选择素（sE-selectin）酶联免疫试剂盒（货号：CSB-E07996r），采用双抗体夹心法原理设计，专用于定量检测血清、血浆、细胞培养上清及组织匀浆样本中的sE-selectin蛋白。E选择素是内皮细胞活化的重要标志物，在炎症反应和免疫调控中发挥关键作用，其可溶性形式可通过血液循环反映病理状态。该试剂盒检测灵敏度为78 pg/mL，线性范围为78-5000 pg/mL，可精准覆盖生理及实验诱导的浓度变化，适用于心血管疾病、糖尿病并发症等炎症相关动物模型的机制研究，或体外细胞实验中内皮细胞激活程度的动态监测。试剂盒包含预包被抗体、标准品及检测试剂，支持多样本类型分析，兼容常规实验室操作流程，为研究炎症信号通路、药物干预效果或组织修复机制提供可靠工具，满足基础科研中对靶标蛋白定量分析的需求。
别名：

Sele ELISA Kit; Elam-1 ELISA Kit; E-selectin ELISA Kit; CD62 antigen-like family member E ELISA Kit; Endothelial leukocyte adhesion molecule 1 ELISA Kit; ELAM-1 ELISA Kit; Leukocyte-endothelial cell adhesion molecule 2 ELISA Kit; LECAM2 ELISA Kit; CD antigen CD62E ELISA Kit
缩写：

SELE
Uniprot No.：

P98105
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

78 pg/mL-5000 pg/mL
灵敏度：

19.5 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat sE-selectin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	92
	Range %	88-108
1:10	Average %	94
	Range %	91-98
1:20	Average %	96
	Range %	89-100
1:40	Average %	84
	Range %	80-88

回收率：

The recovery of rat sE-selectin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	93-105
EDTA plasma (n=4)	94	90-98

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
5000	2.746	2.645	2.696	2.542
2500	1.976	1.875	1.926	1.772
1250	1.228	1.204	1.216	1.062
625	0.782	0.761	0.772	0.618
312	0.522	0.511	0.517	0.363
156	0.363	0.352	0.358	0.204
78	0.290	0.285	0.288	0.134
0	0.154	0.153	0.154

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Hypolipidemic and anti-inflammatory effects of aorta and heart tissues of cattle and pigs in the atherosclerosis rat model Chernukha IM.et al,Anim Sci J,2018
Impact of age on cardiovascular function, inflammation, and oxidative stress in experimental asphyxial cardiac arrest Secher N.et al,Acta Anaesthesiol Scand.,2017
Impaired NO-mediated vasodilatation in rat coronary arteries after asphyxial cardiac arrest. Troelsen TT.et al,Acta Anaesthesiol Scand.,2015
Impaired NO-mediated vasodilatation in rat coronary arteries after asphyxial cardiac arrest T. T. TROELSEN et al,Anesthesia & Pain Management,2015
Effects of interactions between various fats and active/passive phases on postprandial inflammation in rats H. G&uumll Otlu. et al,Biological Rhythm Research,2016
Preserved cerebral microcirculation after cardiac arrest in a rat model Secher N. et al,Microcirculation,2015
Myo-inositol is a promising treatment for the prevention of ovarian hyperstimulation syndrome (OHSS): an animal study Turan GA. et al,Arch Gynecol Obstet,2015
Long-term high-fat consumption leads to downregulation of Akt phosphorylation of eNOS at Ser1177 and upregulation of Sirtuin-1 expression in rat cavernous tissue Tomada I et al,Age (Dordr),2013
Electrical Vagus Nerve Stimulation Attenuates Systemic Inflammation and Improves Survival in a Rat Heatstroke Model Yamakawa K et al,PLoS One,2013

显示更多

收起更多

功能：

Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with SELPLG/PSGL1. May have a role in capillary morphogenesis.
基因功能参考文献：
1. The e-selectin/biliverdin reductase pathway participates in the inhibitory process of hypoxia in pulmonary artery endothelial cell apoptosis which is mediated by the mitochondrial-dependent apoptosis pathway. PMID: 27033411
2. Decrease in uterine pinopodes and L-selectin ligand MECA-79 expression during uterine receptivity period by testosterone may cause failure of blastocyst implantation. PMID: 24966906
3. E-selectin blockade similarly inhibits the migration of activated CCR4(negative) and CCR4(positive) CD4 T cells to dermal inflammation. PMID: 22664869
4. The expression of E-selectin is significantly increased in lung tissue of ovariectomized rats, an effect prevented by estradiol but not by progesterone treatment. PMID: 20735828
亚细胞定位：

Cell membrane; Single-pass type I membrane protein.
蛋白家族：

Selectin/LECAM family
数据库链接：

KEGG: rno:25544

STRING: 10116.ENSRNOP00000034797

UniGene: Rn.10359

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.