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  4. Rat selenoprotein P, plasma, 1 (SEPP1)ELISA kit

Rat selenoprotein P, plasma, 1 (SEPP1)ELISA kit

文献引用 (1) 说明书下载
  • 中文名称：
    大鼠硒蛋白P(SEPP1)酶联免疫试剂盒
  • 货号：
    CSB-E17683r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO定量检测大鼠血清、血浆及组织匀浆样本中硒蛋白P（SEPP1）含量的双抗体夹心法ELISA试剂盒，适用于科研领域对硒蛋白代谢调控机制的研究。SEPP1是一种由肝脏分泌的多功能硒转运蛋白，通过结合硒元素参与抗氧化防御、炎症调节及代谢稳态维持等生理过程。试剂盒检测灵敏度达78 pg/mL，线性范围为78-5000 pg/mL，可在标准曲线范围内精确测定目标蛋白含量，操作流程包含样本预处理、孵育结合、洗涤及显色检测步骤，实验周期约4.5小时。该试剂盒适配多种生物样本类型，尤其适用于探究肝脏疾病模型、营养性硒缺乏或氧化应激相关实验中SEPP1的动态变化，为研究硒元素代谢通路、抗氧化系统功能以及相关疾病分子机制提供可靠的检测工具，广泛应用于基础医学、营养学及分子生物学领域的实验需求。
  • 别名：
    Selenop ELISA Kit; Selp ELISA Kit; Sepp1 ELISA Kit; Selenoprotein P ELISA Kit; SeP) [Cleaved into: Selenoprotein Se-P10; Selenoprotein Se-P6; Selenoprotein Se-P2; Selenoprotein Se-P1] ELISA Kit
  • 缩写：
    SEPP1
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    78 pg/mL-5000 pg/mL
  • 灵敏度：
    19.5 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat SEPP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1000 Average % 92    
    Range % 88-96    
    1:2000 Average % 105    
    Range % 100-110    
    1:4000 Average % 96    
    Range % 92-100    
    1:8000 Average % 89    
    Range % 84-93    
  • 回收率：
    The recovery of rat SEPP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 98 94-102    
    EDTA plasma (n=4) 85 80-90    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    pg/ml OD1 OD2 Average Corrected    
    5000 2.522 2.496 2.509 2.354    
    2500 1.949 2.023 1.986 1.831    
    1250 1.613 1.584 1.599 1.444    
    625 1.186 1.135 1.161 1.006    
    312 0.754 0.721 0.738 0.583    
    156 0.448 0.460 0.454 0.299    
    78 0.315 0.297 0.306 0.151    
    0 0.151 0.158 0.155 　    
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

引用文献

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Selenop Proteins

靶点详情

  • 功能：
    Might be responsible for some of the extracellular antioxidant defense properties of selenium or might be involved in the transport of selenium. May supply selenium to tissues such as brain and testis.
  • 基因功能参考文献：
    1. A chromatin immunoprecipitation (ChIP) assay revealed that EPA decreases binding of SREBP-1c to the SELENOP promoter. Knockdown of Srebf1 resulted in a significant down-regulation of Selenop expression. PMID: 28465347
    2. Data indicate that Selenoprotein P SEPP1 processivity is intrinsic, but efficiency is governed by selenium levels. PMID: 25063811
    3. metformin decreases binding of FoxO3a, a direct target of AMPK, to the SEPP1 promoter. PMID: 24257750
    4. Sepp1 secreted from epithelial cells may support the intestinal immune system by providing immune cells (including plasma cells) with selenium PMID: 24157689
    5. apoER2-mediated uptake of long isoform Sepp1 is responsible for selenium distribution to tissues throughout the body. PMID: 22761431
    6. investigation of Sepp1 expression in adipocytes during adipogenesis and oxidative stress in models of obesity and insulin resistance PMID: 20959537
    7. tear SeP is a key molecule to protect the ocular surface cells against environmental oxidative stress PMID: 20360971
    8. mass spectrometric characterization of full-length protein and three isoforms shortened at the C terminus PMID: 11821412
    9. selenenylsulfide linkages Sec304-Cys314 and Cys306-Sec316 were present; selenoprotein P contains these two selenenylsulfide bonds PMID: 15712351
    10. Sertoli cell ApoER2 is a Sepp1 receptor and a component of the selenium delivery pathway to spermatogenic cells PMID: 17314095
    11. In conclusion, the selenoprotein P promoter is a target of the Akt/FoxO signal transduction cascade and SeP expression is regulated at the level of transcription by the forkhead box protein FoxO1a in human and rat hepatoma cells. PMID: 17986386

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Selenoprotein P family
  • 组织特异性：
    Widely expressed, mainly by the liver. Secreted in plasma.
  • 数据库链接：

    KEGG: rno:29360

    UniGene: Rn.1451

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九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |