Rat secreted phospholipase A2,sPLA2 ELISA Kit

产品详情

产品描述：

大鼠分泌型磷脂酶A2(sPLA2)酶联免疫试剂盒（CSB-E13206r）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的sPLA2含量。sPLA2即分泌型磷脂酶 A2，在生物体内广泛存在。它参与磷脂代谢，能水解膜磷脂产生花生四烯酸和溶血磷脂等。其异常表达与多种疾病如炎症、心血管病、肿瘤等相关，因此针对 sPLA2 作用机制的研究，能为疾病治疗提供新靶点与思路。试剂盒检测范围为0.78 ng/mL-50 ng/mL，支持科研人员探究sPLA2在疾病模型中的动态变化，例如心血管疾病机制研究、炎症相关动物实验或药物干预效果评估，尤其适用于分析血清/血浆样本的循环sPLA2水平或组织匀浆中的局部表达差异本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

sPLA2
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.78 ng/mL-50 ng/mL
灵敏度：

0.195 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat sPLA2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-95
1:2	Average %	93
	Range %	87-97
1:4	Average %	91
	Range %	85-97
1:8	Average %	92
	Range %	88-96

回收率：

The recovery of rat sPLA2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	87	82-93
EDTA plasma (n=4)	92	87-94

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.