Rat relaxin 1 (RLN1) ELISA kit

Rat relaxin 1 (RLN1) ELISA kit

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中文名称：

大鼠松弛肽/松弛素1(RLN1)酶联免疫试剂盒
货号：

CSB-EL019749RA
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

大鼠松弛肽/松弛素1(RLN1)酶联免疫试剂盒（CSB-EL019749RA）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的RLN1含量。试剂盒检测范围为2.4 pg/mL-150 pg/mL，适用于体外实验环境下研究RLN1在生殖生理、心血管疾病模型或组织再生机制中的表达变化，为探索其调控网络及分子互作提供可靠数据支持本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

Rln1 ELISA Kit; Rln ELISA Kit; Prorelaxin 1 [Cleaved into: Relaxin B chain; Relaxin A chain] ELISA Kit
缩写：

RLN1
Uniprot No.：

P01347
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates, cell lysates
检测范围：

2.4 pg/mL-150 pg/mL
灵敏度：

0.6 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat RLN1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	90-99
1:2	Average %	89
	Range %	84-93
1:4	Average %	92
	Range %	88-96
1:8	Average %	97
	Range %	92-101

回收率：

The recovery of rat RLN1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	105	100-110
EDTA plasma (n=4)	84	80-88

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
150	2.643	2.487	2.565	2.439
75	2.097	2.236	2.167	2.041
37.5	1.468	1.551	1.510	1.384
18.75	0.907	0.945	0.926	0.800
9.4	0.583	0.617	0.600	0.474
4.7	0.384	0.401	0.393	0.267
2.4	0.235	0.252	0.244	0.118
0	0.121	0.130	0.126

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Effects of swimming exercise and RLN2 induction on relaxin levels, MMP-13, TGFβ expression in the pubic cartilage of pregnant rats I Oktaviani,Journal of Pharmacy & Pharmacognosy Research,2022

功能：

Relaxin is an ovarian hormone that acts with estrogen to produce dilatation of the birth canal in many mammals.
基因功能参考文献：
1. Relaxin-1 mRNA in the hearts of diabetic rats was significantly increased and that relaxin-3 mRNA expression at different time points was decreased. In addition, significant down-regulation of the mRNA expression of RXFP1 and RXFP3 was observed at 4 weeks after Streptozocin. PMID: 28776188
2. The digestive tract has been shown to express relaxin receptors; the hormone appears to exert site-specific effects acting at the neural or at the smooth muscle level, mainly by a nitric oxide-mediated mechanism. (Review) PMID: 28606038
3. We have shown that relaxin stimulates Sertoli cell proliferation through the activation of MEK/ERK1/2 and PI3K/AKT pathways. PMID: 24640565
4. The aim of the present study was to explore a role of relaxin at specific stages of spermatogenesis using a co-culture of rat Sertoli and germ cells. PMID: 24640566
5. Raxin is a mediator of the refractoriness of the mesangial cells to angiotensin II during pregnancy. PMID: 23207895
6. Data demonstrate a functional role for relaxin in mediating uterine artery compliance in pregnant rats, which may be necessary to maintain adequate uterine blood flow to the uterus and placenta. PMID: 22744867
7. A novel G-quadruplex in the relaxin gene, is reported. PMID: 22363579
8. Rln-1 was localized in the granulosa cells of the ovarian follicles, and immunoreactivity against Rln 1 was increased by orthodontic tooth movement in a time-dependent manner. PMID: 21410550
9. relaxin regulates fibroblast proliferation, differentiation, and collagen deposition and may have therapeutic potential in diseased states characterized by cardiac fibrosis. PMID: 15155573
10. role for relaxin in the systemic hemodynamic changes of pregnancy, as well as novel therapeutic potential for relaxin in modifying arterial stiffness and cardiac afterload. PMID: 15198972
11. High glucose concentration could stimulate the expression of endogenous relaxin. PMID: 19542742
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亚细胞定位：

Secreted.
蛋白家族：

Insulin family
数据库链接：

KEGG: rno:25616

STRING: 10116.ENSRNOP00000021315

UniGene: Rn.9830

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.