Rat platelet membrane glycoprotein ⅡbⅢa,GP-ⅡbⅢa ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

大鼠血小板膜糖蛋白ⅡbⅢa(GP-ⅡbⅢa/CD41+CD61)酶联免疫试剂盒（CSB-E08776r）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的GPⅡbⅢa/CD41+CD61含量。GPⅡbⅢa/CD41+CD61作为靶点，在血小板功能中至关重要。参与血小板聚集过程，研究机制聚焦于通过阻断该靶点，抑制血小板的聚集与黏附，进而为抗血栓药物研发提供方向，以防治相关心血管疾病。试剂盒检测范围为6.25 ng/mL-400 ng/mL，适用于多种生物样本中GP - ⅡbⅢa的精确检测，可广泛用于大鼠模型中血小板功能调控机制研究、血栓相关疾病动物模型的病理分析、药物干预效果评价等科研领域，为探索血小板相关分子机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

platelet membrane glycoprotein ⅡbⅢa,GP-ⅡbⅢa |platelet membrane glycoprotein 2b3a,GP-2b3a
缩写：

GPⅡbⅢa/CD41+CD61
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

6.25 ng/mL-400 ng/mL
灵敏度：

1.56 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat GP-ⅡbⅢa in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	82-92
1:2	Average %	89
	Range %	82-95
1:4	Average %	95
	Range %	90-100
1:8	Average %	90
	Range %	85-98

回收率：

The recovery of rat GP-ⅡbⅢa spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-97
EDTA plasma (n=4)	98	92-105

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.