Rat pigment epithelium-derived factor,PEDF ELISA Kit

Rat pigment epithelium-derived factor,PEDF ELISA Kit

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中文名称：

大鼠色素上皮衍生因子(PEDF)酶联免疫试剂盒
货号：

CSB-E08819r
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

大鼠色素上皮衍生因子(PEDF)酶联免疫试剂盒（CSB-E08819r）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的PEDF含量。PEDF（色素上皮衍生因子）是一种具有多种生物功能的分泌性糖蛋白，主要在视网膜色素上皮细胞中表达。它具有抗血管生成、抗炎和神经营养作用，对多种肿瘤细胞具有抑制作用。其研究机制涉及通过调节细胞增殖、凋亡和迁移等途径抑制肿瘤生长。试剂盒检测范围为6.25 pg/mL-400 pg/mL，适用于体外培养细胞分泌功能评估、疾病模型动物血清标志物分析以及组织微环境中PEDF动态变化的科研探索，为血管生成调控、氧化应激机制及神经退行性疾病等基础研究提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

PEDF
Uniprot No.：

Q80ZA3
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

6.25 pg/mL-400 pg/mL
灵敏度：

1.56 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cancer
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat PEDF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	87
	Range %	81-92
1:2000	Average %	95
	Range %	91-99
1:4000	Average %	92
	Range %	85-97
1:8000	Average %	96
	Range %	91-102

回收率：

The recovery of rat PEDFspiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	89-95
EDTA plasma (n=4)	87	83-91

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
400	2.758	2.615	2.687	2.549
200	2.065	1.968	2.017	1.879
100	1.357	1.397	1.377	1.239
50	0.846	0.831	0.839	0.701
25	0.572	0.581	0.577	0.439
12.5	0.354	0.334	0.344	0.206
6.25	0.264	0.256	0.260	0.122
0	0.139	0.137	0.138

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Application of rat cryptorchidism model for the evaluation of mesenchymal stromal cell secretome regenerative potential G.D.Sagaradze, et al,Biomedicine & Pharmacotherapy,2018
A novel modified physiologically relevant model for cardiac angiogenesis Hao Zhang.et al,Microvasc Res,2017
Pigment epithelium-derived factor attenuates myocardial fibrosis via inhibiting Endothelial-to-Mesenchymal Transition in rats with acute myocardial infarction Hao Zhang.et al,Scientific Reports,2017
Pigment Epithelium-Derived Factor (PEDF) Improves Ischemic Cardiac Functional Reserve Through Decreasing Hypoxic Cardiomyocyte Contractility Through PEDF Receptor (PEDF-R). Lu P.et al,J Am Heart Assoc.,2016
PEDF and PEDF-derived peptide 44mer inhibit oxygen-glucose deprivation-induced oxidative stress through upregulating PPAR纬 via PEDF-R in H9c2 cells. Wei Zhuang.et al,Biochem Biophys Res Commun.,2016
PEDF and 34-mer inhibit angiogenesis in the heart by inducing tip cells apoptosis via up-regulating PPAR-纬 to increase surface FasL Zhang H. et al,Apoptosis,2015
Comparison of the effects of letrozole and cabergoline on vascular permeability, ovarian diameter, ovarian tissue VEGF levels, and blood PEDF levels, in a rat model of ovarian hyperstimulation syndrome. ?ahin N. et al,Arch Gynecol Obstet,2015
Influence of triazine herbicide exposure on guppies (poecilia sphenops) aromatase activities, altered sex steroid concentration and vitellogenin induction S Vasanth. et al,Indian J Pharm Sci.,2015
PEDF Improves Cardiac Function in Rats with Acute Myocardial Infarction via Inhibiting Vascular Permeability and Cardiomyocyte Apoptosis Zhang H et al,Int J Mol Sci,2015
PEDF and PEDF-derived peptide 44mer stimulate cardiac triglyceride degradation via ATGL Gao X et al,Sci Rep,2014

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.