Rat periostin/osteoblast specific factor 2 (POSTN) ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

大鼠骨膜蛋白/成骨细胞特异性因子2(POSTN)酶联免疫试剂盒（CSB-EL018381RA）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的POSTN含量。POSTN是一个靶点。它与多种生理和病理过程相关，在肿瘤发生、发展中可能起重要作用。研究机制上，关注其在细胞外基质中的功能，以及与肿瘤细胞增殖、迁移、侵袭等的关联，有望为肿瘤等疾病的治疗提供新方向。试剂盒检测范围为31.25 ng/mL-2000 ng/mL，适用于骨骼生物学研究、骨损伤修复机制探索、纤维化疾病模型建立及肿瘤微环境相关研究等科研领域，为体外定量分析POSTN在生理或病理条件下的动态变化提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

POSTN
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

31.25 ng/mL-2000 ng/mL
灵敏度：

7.81 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat POSTN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	86-96
1:2	Average %	104
	Range %	98-108
1:4	Average %	90
	Range %	85-94
1:8	Average %	98
	Range %	92-101

回收率：

The recovery of rat POSTN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	84-95
EDTA plasma (n=4)	98	90-103

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.