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  4. Rat p53/tumor protein,p53/TP53 ELISA Kit

Rat p53/tumor protein,p53/TP53 ELISA Kit

文献引用 (14) 说明书下载
  • 中文名称：
    大鼠P53(P53)酶联免疫试剂盒
  • 货号：
    CSB-E08336r
  • 规格：
    96T/48T
  • 价格：
    ￥3900/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO大鼠P53（P53）酶联免疫检测试剂盒（货号：CSB-E08336r），基于双抗体夹心法定量原理设计，可精准检测血清、血浆及组织匀浆样本中P53蛋白的表达水平。P53作为重要的肿瘤抑制蛋白，广泛参与细胞周期调控、DNA修复及凋亡等生物学过程，是癌症研究、细胞应激反应及药物作用机制探索的关键靶点。本试剂盒检测灵敏度高，线性范围为12.5-800 pg/mL，可满足微量样本的定量需求，实验操作流程优化后可在4.5小时内完成。试剂盒包含预包被捕获抗体的酶标板、检测抗体、标准品及显色底物等核心组分，适用于体外科研场景中P53蛋白的定量分析，例如肿瘤动物模型研究、基因表达调控实验或细胞应激状态下蛋白动态变化的监测。通过严格的抗体配对验证，确保检测结果的特异性与可靠性，为分子机制研究提供稳定工具。
  • 缩写：
    TP53
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    12.5 pg/mL-800 pg/mL
  • 灵敏度：
    3.12 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat p53/TP53 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %100
    Range %95-108
    1:2Average %87
    Range %82-91
    1:4Average %90
    Range %85-94
    1:8Average %92
    Range %88-96
  • 回收率：
    The recovery of rat p53/TP53 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8984-93
    EDTA plasma (n=4)9590-100
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1OD2AverageCorrected
    8002.479 2.615 2.547 2.405
    4002.009 1.974 1.992 1.850
    2001.356 1.330 1.343 1.201
    1000.874 0.929 0.902 0.760
    500.532 0.571 0.552 0.410
    250.401 0.425 0.413 0.271
    12.50.297 0.287 0.292 0.150
    00.140 0.143 0.142
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

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相关问答

■ 常见问题解答
Q:

How can I convert pyggrams per decilitre to picograms per milligram, because your kit is in deciliters and concentrates
picogeram/dl---picogeram/mg.
This kit is according to picogerm/dl, i want according to picogeram /mg.

A:
Thanks for your inquiry. For this kit,convertions are not suggested.We recommend you calculate according to the unit stated in the manual.
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靶点详情

  • 功能：
    Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Its pro-apoptotic activity is activated via its interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2. However, this activity is inhibited when the interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2 is displaced by PPP1R13L/iASPP. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seems to have an effect on cell-cycle regulation. Regulates the circadian clock by repressing CLOCK-ARNTL/BMAL1-mediated transcriptional activation of PER2.
  • 相关疾病：
    p53 is found in increased amounts in a wide variety of transformed cells. p53 is frequently mutated or inactivated in many types of cancer.
  • 亚细胞定位：
    Cytoplasm. Nucleus. Nucleus, PML body. Endoplasmic reticulum. Mitochondrion matrix. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
  • 蛋白家族：
    P53 family
  • 数据库链接：

    KEGG: rno:24842

    STRING: 10116.ENSRNOP00000047840

    UniGene: Rn.54443

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九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |