Rat oncostatin M (OSM) ELISA kit

产品详情

产品描述：

CUSABIO定量检测大鼠血清、血浆中抑瘤素M（OSM）的酶联免疫试剂盒，采用双抗体夹心法原理设计，具有高灵敏度和特异性。OSM是IL-6细胞因子家族成员，在炎症反应、组织修复及免疫调节中发挥重要作用，其表达水平与多种病理生理过程相关。试剂盒检测范围覆盖15.6-1000 pg/mL，最低检测限为3.9 pg/mL，实验全程约需4.5小时，标准品和样本均设置复孔检测以保证结果可靠性。本产品适用于科研领域中对大鼠OSM蛋白表达的定量分析，可应用于炎症性疾病模型、组织再生机制研究或免疫调控相关实验，为探索OSM在心血管疾病、纤维化病变等领域的分子机制提供可靠工具。试剂盒提供预包被酶标板、标准品、检测抗体及全套实验溶液，操作流程标准化，支持96孔板规格批量检测，配套说明书包含详细操作步骤及数据处理方法。
别名：

Osm ELISA Kit; Oncostatin-M ELISA Kit; OSM ELISA Kit
缩写：

OSM
Uniprot No.：

Q65Z15
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma
检测范围：

15.6 pg/mL-1000 pg/mL
灵敏度：

3.9 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat OSM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-95
1:2	Average %	104
	Range %	99-108
1:4	Average %	91
	Range %	85-97
1:8	Average %	95
	Range %	91-99

回收率：

The recovery of rat OSM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	92-102
EDTA plasma (n=4)	89	82-93

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Growth regulator. Inhibits the proliferation of a number of tumor cell lines. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells. Uses only type II OSM receptor (heterodimers composed of OSMR and IL6ST). Involved in the maturation of fetal hepatocytes, thereby promoting liver development and regeneration.
基因功能参考文献：
1. OSM is a key mediator for inducing differentiation of OC15-5 cells into hepatocytes PMID: 15743783
2. In the immortalized mouse and primary cultured proliferative rat hepatocytes, treatment with OSM markedly increased mRNA and protein of claudin-2 together with formation of developed networks of TJ strands. PMID: 17434483
3. OSM may not only play a role in the regulation of Sertoli cell proliferation and the initiation of spermatogenesis but may also play a role in the regulation of Leydig cell progenitor formation. PMID: 17996055
4. Results indicate that osteosarcoma cells stably producing OSM do not develop resistance to this cytokine and thus could be a valuable new tool to study the anti-cancer effect of OSM in vivo. PMID: 19168167
亚细胞定位：

Secreted.
蛋白家族：

LIF/OSM family
组织特异性：

Widely expressed. Expressed at higher levels in liver, skin and spleen.
数据库链接：

KEGG: rno:289747

STRING: 10116.ENSRNOP00000032548

UniGene: Rn.127158