Rat nuclear factor-κB p65,NF-κB p65 ELISA Kit

Rat nuclear factor-κB p65,NF-κB p65 ELISA Kit

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中文名称：

大鼠核因子-κB亚基p65亲和肽(NF-κB p65)酶联免疫试剂盒
货号：

CSB-E08788r
规格：

96T/48T
价格：

￥3900/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO大鼠核因子-κB亚基p65亲和肽（NF-κB p65）酶联免疫吸附检测试剂盒（货号：CSB-E08788r），基于双抗体夹心法原理开发，适用于定量检测大鼠血清、血浆及组织匀浆样本中p65亚基的蛋白表达水平。NF-κB是调控炎症反应、免疫应答及细胞存活的关键转录因子，其p65亚基在信号转导中发挥核心作用，其异常表达与多种病理过程相关。本试剂盒检测灵敏度为1.56 pg/mL，线性检测范围为1.56-100 pg/mL，可精准捕捉低浓度样本中的靶标变化。试剂盒配套标准品、预包被抗体及检测所需试剂，操作流程优化，适用于基础科研中炎症机制研究、免疫调控实验或药物干预效果评估，例如在动物模型中分析NF-κB信号通路激活状态，或探究氧化应激、感染性疾病等实验条件下p65亚基的动态表达。检测样本兼容性强，支持多种生物液体及组织样本处理，为分子机制研究提供可靠工具。
别名：

NF-Kappa B,nuclear factor-kappa B
缩写：

NFκB p65
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

1.56 pg/mL-100 pg/mL
灵敏度：

0.39 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat NF-κB p65 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	89
	Range %	86-92
1:200	Average %	99
	Range %	97-103
1:400	Average %	89
	Range %	85-93
1:800	Average %	95
	Range %	91-99

回收率：

The recovery of rat NF-κB p65 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	91-97
EDTA plasma (n=4)	94	90-98

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
100	2.039	2.022	2.031	1.887
50	1.334	1.326	1.330	1.186
25	0.908	0.902	0.905	0.761
12.5	0.566	0.557	0.562	0.418
6.25	0.358	0.364	0.361	0.217
3.12	0.291	0.281	0.286	0.142
1.56	0.243	0.232	0.238	0.094
0	0.145	0.143	0.144

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Targeting TLR4/NF-κB signaling, oxidative stress, and apoptosis by farnesol mitigates cadmium-induced testicular toxicity in rats EHM Hassanein, MF Alotaibi, RS Alruhaimi, M Sabry,Tissue & Cell,2025
Swertiamarin ameliorates cognitive dysfunction by improving hyperglycemia and neuroinflammation in type 2 diabetic rats via activation of the PI3K/AKT/GSK3β signaling pathway B Tan, L Liu, T Wu, F Yuan, C Wang,Central European Journal of Immunology,2024
The sesquiterpene alcohol farnesol mitigates cadmium hepatotoxicity by attenuating oxidative stress and NF-κB/NLRP3 inflammasome axis and upregulating PPARγ in rats RS Alruhaimi, EHM Hassanein, SM Alnasser,EXCLI Journal,2024
Green Synthetized Selenium Nanoparticles Using Syzygium aromaticum (Clove) Extract Reduce Pentylenetetrazol-Induced Epilepsy and Associated Cortical Damage in Rats MS Othman,Applied Sciences,2023
Rosmarinic acid mitigates chlorpyrifos-induced oxidative stress, inflammation, and kidney injury in rats by modulating SIRT1 and Nrf2/HO-1 signaling MS Abduh,Life sciences,2022
Hydrogen gas ameliorates the LPS-induced BPD via inhibiting the activation of TNF-α/NF-κB inflammatory signaling pathway in placenta Y Zhang,researchsquare,2022
Reno-protective effect of mangiferin against methotrexate-induced kidney damage in male rats: PPARγ-mediated antioxidant activity SM Elshazly,Saudi Pharmaceutical Journal,2022
Apigenin attenuates molecular, biochemical, and histopathological changes associated with renal impairments induced by gentamicin exposure in rats Y Xie,ResearchSquare,2022
Inosine mitigated diabetic peripheral neuropathy via modulating GLO1/AGEs/RAGE/NF-κB/Nrf2 and TGF-β/PKC/TRPV1 signaling pathways NF Abdelkader,Biomedecine & pharmacotherapie,2021
Postconditioning attenuates myocardial ischemia‑reperfusion injury by inhibiting complement activation and upregulation of miR‑499 Z Huang,Experimental and Therapeutic Medicine,2021
Alogliptin attenuates lipopolysaccharide-induced neuroinflammation in mice through modulation of TLR4/MYD88/NF-κB and miRNA-155/SOCS-1 signaling pathways Ayman E El-Sahar,International Journal of Neuropsychopharmacology,2020
Suppression of inflammatory cascades via novel cinnamic acid nanoparticles in acute hepatitis rat model EA Ibrahim,Archives of Biochemistry and Biophysics,2020
Regulation of liver regeneration by prostaglandin E 2 and thromboxane A 2 following partial hepatectomy in rats YS Mohamed,Naunyn-Schmiedeberg\'s Archives of Pharmacology,2020
Vitamin D3 Modulates NF-kB/p65, 17β-Estradiol, and Vitamin D Receptors Expression at Estrogen Deficiency Koshkina A, et al,Vitamin D Defjciency,2019
Inflammatory Consequences of Maternal Diabetes on the Offspring Brain: a Hippocampal Organotypic Culture Study Glombik K,Neurotoxicity Research,2019
Hepato-protective Effect of Ultrasonicated Ginseng Berry Extract on Rat Mild Bile Duct Ligation Model Yoonjin Nam.et al,Journal of Ginseng Research,2018
Involvement of the NF-魏B signaling pathway in the renoprotective effects of isorhamnetin in a type 2 diabetic rat model Shujuan Qiu.et al,Biomedical Reports,2016

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.