Rat neuropeptide Y (NPY) ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO大鼠神经肽Y（NPY）酶联免疫检测试剂盒（货号：CSB-E13431r），采用双抗体夹心法检测原理，可定量分析血清、血浆及组织匀浆样本中NPY的浓度，线性检测范围为1.56 pg/mL至100 pg/mL。神经肽Y是一种广泛分布于中枢及周围神经系统的生物活性肽，参与调控能量代谢平衡、摄食行为及应激反应等生理过程，其水平变化与神经系统功能及代谢性疾病密切相关。本试剂盒通过特异性捕获抗体和酶标记检测抗体的协同作用，实现目标蛋白的高灵敏度检测，适用于神经生物学研究、代谢调节机制探索、药物干预效果评价等科研领域。实验操作流程标准化，兼容常规实验室设备，能够稳定检测低丰度样本，支持大鼠模型中的NPY动态监测，例如神经退行性疾病模型、肥胖症研究或应激反应实验中的生物标志物分析。试剂盒组分包含预包被酶标板、标准品、检测抗体及显色底物等，需自备样本稀释液和洗板缓冲液。
缩写：

NPY
Uniprot No.：

P07808
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

1.56 pg/mL-100 pg/mL
灵敏度：

0.39 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Neuroscience
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat NPY in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	92-104
1:2	Average %	97
	Range %	92-103
1:4	Average %	93
	Range %	89-95
1:8	Average %	86
	Range %	82-92

回收率：

The recovery of rat NPY spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	87-99
EDTA plasma (n=4)	105	100-110

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

NPY is implicated in the control of feeding and in secretion of gonadotrophin-release hormone.
亚细胞定位：

Secreted. Cytoplasmic vesicle, secretory vesicle, neuronal dense core vesicle.
蛋白家族：

NPY family
组织特异性：

One of the most abundant peptides in the nervous system. Also found in some chromaffin cells of the adrenal medulla.
数据库链接：

KEGG: rno:100912228

STRING: 10116.ENSRNOP00000013146

UniGene: Rn.9714